银杏内酯B对星形胶质细胞释放NO、IL-6及趋化因子RANTES的影响

被引：7

作者：

彭珊瑛 ^{[1
]}

廖文辉 ^{[1
]}

聂珍贵 ^{[2
]}

刘洋 ^{[1
]}

王霖 ^{[1
]}

王凤 ^{[1
]}

王文杰 ^{[1
]}

机构：

[1] 中国医学科学院、北京协和医学院药物研究所

[2] 首都医科大学燕京医学院

来源：

药学学报 | 2010年 / 09期

基金：

国家高技术研究发展计划(863计划);

关键词：

银杏内酯B; NO; IL-6; RANTES; 星形胶质细胞;

D O I：

10.16438/j.0513-4870.2010.09.002

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

考察银杏内酯B对星形胶质细胞释放NO、IL-6及趋化因子RANTES的影响,探讨在炎性刺激条件下,银杏内酯B对星形胶质细胞的保护作用机制。采用Griess试剂检测培养上清液中NO的含量,ELISA法测定IL-6及RANTES的含量,RT-PCR法测定IL-6及RANTESmRNA的表达。结果显示,LPS可显著刺激大鼠原代星形胶质细胞释放NO,银杏内酯B可显著抑制LPS刺激的NO释放;IL-1β可显著刺激U251星形胶质瘤细胞IL-6和RANTES的生成,并增加其mRNA的表达,银杏内酯B可明显抑制IL-1β诱导的IL-6和RANTES的生成,并可显著抑制IL-1β和RANTESmRNA的表达。因此,银杏内酯B可能是通过减少NO的生成,降低星形胶质细胞炎症因子IL-6mRNA和细胞趋化因子RANTESmRNA的表达,从而减少IL-6及RANTES的产生,减轻炎症反应的程度。结果表明,银杏内酯B可能通过降低AD发病和进展过程中的与星形胶质细胞相关的慢性炎症反应,从而缓解AD症状。

引用

页码：1103 / 1108

页数：6

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