褐脉少花龙葵毛状根的诱导、培养及其澳洲茄胺的产生

被引：7

作者：

吴晓凤 ^{[1
]}

施和平 ^{[1
]}

TSANG Po Keung Eric ^{[2
]}

机构：

[1] 华南师范大学生命科学学院广东省植物发育生物工程实验室

[2] 香港教育学院数社科学系

来源：

分子细胞生物学报 | 2008年 / 03期

关键词：

发根农杆菌; 褐脉少花龙葵; 毛状根; 澳洲茄胺; 氮源; 钙;

D O I：

暂无

中图分类号：

S567.239 [];

学科分类号：

1008 ;

摘要：

利用发根农杆菌的遗传转化和液体培养技术,研究了褐脉少花龙葵(Solanum nigrum L. var. pauciflorum)毛状根的诱导和离体培养及其澳洲茄胺的产生以及液体培养过程中培养基中N源和钙的消耗变化。结果表明,发根农杆菌ATCC15834感染褐脉少花龙葵叶片外植体5 d后产生毛状根,感染25d后,约90%的叶片外植体产生毛状根。毛状根能在无外源生长调节剂的MS固体和液体培养基上自主生长。PCR扩增结果显示发根农杆菌Ri质粒的rolB和rolC基因已在少花龙葵毛状根基因组中整合并得到表达。所产生的毛状根能产生药用次生物质澳洲茄胺,其含量约为非转化植株根的1.3倍,达到582.05μg/g干重。少花龙葵毛状根液体培养0-5 d内处于生长迟滞期、5-15 d为快速生长期、15d后进入生长平台期。培养基的硝态氮和铵态氮在毛状根液体培养过程中被逐渐吸收和消耗,至培养15 d时铵态氮被消耗殆尽,而硝态氮仍剩余44.7%;培养基中钙的浓度在培养过程中虽逐渐降低,但在培养25d时仍未被完全消耗,其浓度约为起始浓度的43.5%。该结果为今后设计合适的培养基来规模培养褐脉少花龙葵毛状根生产药用次生物质澳洲茄胺提供了可能性。

引用

页码：183 / 191

页数：9

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