THE EFFECTS OF NITROGEN,PHOSPHORUS,VITAMINS AND TRACE METALS ON THE GROWTH OF THE RED TIDE ORGANISM PROROCENTRUM MICANS

Laboratory culture experiments showed that < 100μmol/L nitrate, amonium or mixture of aminoacids promote the growth of the red tide organism Prorocentrum micans Ehrenb, but that >100μmol/Lof ammonium, or mixture of glycine and glutamate was harmful to growth, and that orthophosphatewas P. micrns’main phosphorous soruce in the ocean. Presence of 80μmol/L EDTA, 0.5 to 1μmol/LFe, 1.0 to 20.0 μmol/L Mn0.1 to 0.4 μmol/L Coin the culture medium could improvethe growth of P. micans. Vitamin Bpromoted growth, but vitamin Band biotin did not. Theestimated minimum cell quotaS （q） for nitrogen and phosphorus being 0.74 pmole/cell and 0.045pmole/cell show that phosphorus （more than nitrogen） limits the growth of P. micans in the study area.