白藜芦醇调节STAT3与miR-21表达抗肝癌作用的初步研究

被引：17

作者：

李覃 ^{[1
]}

王伟 ^{[2
]}

张实 ^{[3
]}

邢杰 ^{[1
]}

王增田 ^{[4
]}

机构：

[1] 天津武警后勤学院病原生物与免疫学教研室

[2] 天津武警后勤学院附属医院

[3] 天津武警后勤学院实验动物中心

[4] 天津武警后勤学院临床医学系

来源：

中国药理学通报 | 2014年 / 30卷 / 02期

基金：

中国博士后科学基金;

关键词：

白藜芦醇; 肝癌; 调节性T细胞; 信号转导; miR-21; STAT3;

D O I：

暂无

中图分类号：

R735.7 [肝肿瘤];

学科分类号：

100112 [医学生物化学与分子生物学];

摘要：

目的初步探讨白藜芦醇(Resveratrol,Res)的抗肝癌作用及其分子机制。方法建立移植性肝癌小鼠模型,口服给予Res进行干预,分析肿瘤生长状况。然后采用Real-time PCR、RT-PCR等方法检测转录因子Foxp3及微小核糖核酸-21(miR-21)的基因表达,Western blot分析信号转导子与转录激活子3(STAT3)的活性表达,流式细胞术观察CD4+CD25+T细胞数量的变化。结果与荷瘤对照组相比,Res给药组CD4+CD25+T细胞数量和Foxp3表达均明显降低,同时miR-21表达减少,该效应或许与其下调STAT3磷酸化活性水平密切相关。结论 Res可能通过调控STAT3信号分子及miR-21表达,抑制调节性T细胞产生,改变肿瘤微环境,从而发挥抗肝癌作用。

引用

页码：186 / 191

页数：6

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