洛伐他汀能下调人脐静脉内皮细胞血凝素样氧化型低密度脂蛋白受体mRNA的表达(英文)

被引：7

作者：

全智华

李全忠

邓衡

易光辉

杨永宗

机构：

[1] 南华大学附属第一医院心内科

[2] 南华大学心血管病研究所

来源：

中国动脉硬化杂志 | 2002年 / 01期

关键词：

洛伐他汀; 内皮,血管; 脂蛋白,低密度; 受体,脂蛋白,低密度;

D O I：

暂无

中图分类号：

R540.2 [];

学科分类号：

摘要：

目的　探讨洛伐他汀对人脐静脉内皮细胞表达血凝素样氧化型低密度脂蛋白受体mRNA和摄取氧化型低密度脂蛋白的影响。方法　用TRIzol试剂常规方法提取人脐静脉内皮细胞总RNA ,逆转录聚合酶链反应检测血凝素样氧化型低密度脂蛋白受体mRNA含量 ,检测12 5　I标记的氧化型低密度脂蛋白放射活性法来确定内皮细胞摄取率 ,分光光度法测量乳酸脱氢酶含量。结果　人脐静脉内皮细胞与 1、10和 5 0 μmol/L洛伐他汀孵育 4 8h后 ,血凝素样氧化型低密度脂蛋白受体mRNA的表达分别为对照组的 84 %、6 9%和 4 8% ;与 10 μmol/L洛伐他汀孵育 2 4、4 8和 96h后 ,血凝素样氧化型低密度脂蛋白受体mRNA的表达分别为对照组的 82 %、6 9%和 5 2 %。洛伐他汀使人脐静脉内皮细胞对氧化型低密度脂蛋白的摄取减少 ,对乳酸脱氢酶含量没有影响。结论　洛伐他汀能降低血凝素样氧化型低密度脂蛋白mRNA的表达和对氧化型低密度脂蛋白的摄取。

引用

页码：26 / 29

页数：4

共 12 条

[1]

Nagase M,Hirose S,Sawamura T,et al.Enhanced expression of endothelial oxidized low-density lipoprotein receptor (LOX-1) in hypertensive rats. Biochemical and Biophysical Research Communications . 1997

[2]

Kataoka H,Kume N,Miyamoto S,et al.Expression of lectinlike oxidized low density lipoprotein receptor-1 in human atherosclerotic lesions. Circulation . 1999

[3]

Li D,Mehta JL.Upregulation of endothelial receptor for oxidized LDL (LOX-1)by oxidized LDL and implications in apoptosis of human coronary artery endothelial cells: evidence from use of antisense LOX-1 mRNA and chemical inhibitors. Arteriosclerosis and Thrombosis . 2000

[4]

Witzum JL,Steinberg D.Role of oxidized low density lipoprotein in atherogenesis. The Journal of Clinical Investigation . 1991

[5]

Chen M,Kakutani M,Minami M,et al.Increased expression of lectin-like oxidized low density lipoprotein receptor-1in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits. Arteriosclerosis and Thrombosis . 2000

[6]

Kume N,Cybulsky MI,Gimbrone MA Jr.Lysophosphatidylcholine, a component of atherogenic lipoproteins, induces mononuclear leukocyte adhesion molecules in cultured human and rabbit arterial endothelial cells. The Journal of Clinical Investigation . 1992

[7]

Sawamura T,Kume N,Aoyama T,et al.An endothelial receptor for oxidized low-density lipoprotein. Nature . 1997

[8]

O’Driscoll G,Green D,Taylor RR.Simvastatin, an HMG-coenzyme A reductase inhibitor, improves endothelial function within 1 month. Circulation . 1997

[9]

Murase T,Kume N,Korenaga R,et al.Fluid shear stress transcriptionally induces lectin-like oxidized LDL receptor-1 in vascular endothelial cells. Circulation Research . 1998

[10]

Kume N,Gimbrone MA Jr.Lysophosphatidylcholine transcriptionally induces growth factor gene expression in cultured human endothelial cells. The Journal of Clinical Investigation . 1994

← 1 2 →