成人胰腺干细胞转分化为胰岛细胞过程中的形态学观察

被引:16
作者
宋振顺
顾克菊
机构
[1] 第四军医大学西京医院肝胆外科
[2] 第四军医大学西京医院感染管理科
关键词
胰腺干细胞; 胰岛; 转分化; 形态学;
D O I
暂无
中图分类号
R329.4 [人体器官组织学(显微解剖学、组织解剖学)];
学科分类号
100101 ;
摘要
目的 :观察成人胰腺干细胞转分化为胰岛过程中的形态学变化 ,以便更深入了解有关机制。方法 :人胰腺组织经胶原酶消化后 ,用梯度离心法将胰腺外分泌细胞和胰岛分离 ,混于外分泌组织中的导管上皮细胞即具有转分化潜能的干细胞。在体外经CMRL10 6 6及不含血清的DMEM /F12加多种营养因子的培养液中培养 2 7d ,在培养的不同时间点取细胞作PDX 1、CK 19等单抗的免疫组化染色。光镜和电镜观察不同时间点的细胞形态学变化。结果 :上述方法可获得大量以往在胰岛分离时丢弃的胰腺导管上皮细胞。经体外一定条件的培养后 ,其形态学变化过程为 :导管上皮细胞迅速分裂增殖转变为有分化能力的干细胞继而转分化为胰岛细胞。结论 :成人胰腺的导管上皮具有干细胞潜能 ,并可在体外转分化为大量具有内分泌功能的胰岛。用此方法获得大量的胰岛可能为克服胰岛移植的供体短缺提供一条新的途径
引用
收藏
页码:196 / 200
页数:5
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