影响纤维素酶解的因素和纤维素酶被吸附性能的研究

被引:34
作者
陈洪章
李佐虎
机构
[1] 中国科学院化工冶金研究所生化工程国家重点实验室!北京
关键词
纤维素; 酶解机制; 汽爆麦草; 结晶纤维素;
D O I
暂无
中图分类号
Q814 [酶工程];
学科分类号
082203 ;
摘要
本文以预处理的木质纤维素和结晶纤维素为材料 ,研究纤维素的吸附过程及影响酶解的主要因素 ,进而探讨纤维素酶解机制。纤维素酶的被吸附过程表现周期吸附和脱吸过程 ,纤维素吸附酶和非吸附酶对汽爆麦草和微晶纤维素酶解有增强效应。半纤维素、木质素对纤维素酶具有同样的吸附性 ,使之无效增加纤维素酶制剂的用量。并提出了天然纤维素酶解过程可分三个阶段。首先是纤维素对纤维素酶的可及性 ;其次是纤维素酶的被吸附与扩散过程 ;最后是由 CBH,CMCase和 βGase自组织复合体 (C1 )协同作用降解纤维素的结晶区 ,同时由 CBH,CMCase和 βGase随机作用纤维素的无定形区。
引用
收藏
页码:30 / 35+71 +71
页数:7
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