PC 12细胞定性及定量测定神经生长因子活性的新方法

被引:8
作者
董跃伟
徐天瑞
熊郁良
徐康森
机构
[1] 云南省药品检验所!昆明
关键词
PC12细胞; 神经生长因子; 活性测定;
D O I
10.13375/j.cnki.wcjps.1999.z1.005
中图分类号
R927.1 [药物检验];
学科分类号
1007 ;
摘要
对用PC12 细胞培养测定神经生长因子活性的方法进行了探索,通过对几种培养条件的比较,找出了较为理想的能定性及定量测定神经生长因子的两种方法,为神经生长因子作为新药检测手段提供了一条新途径。
引用
收藏
页码:311 / 314
页数:4
相关论文
共 6 条
[1]  
Mutationalstudies ofconserved residues in the dim erinterface ofnerve growthfactor. Guo I,Ieyer SL,Kaur H,etal. Protein Science . 1996
[2]  
Nerve growth factor inducedprocess form ation of cultured rat pheochrom ocytom acells. Tischler AS,Greene LA. Nature . 1975
[3]  
A quantitative bioassay for never growthfactoractivity em ploying a clonalpheochromocytom a cellline. Greene LA. Brain Research . 1977
[4]  
Establishm ent of anoradrenergicclonallineofratadrenalpheochrom ocytom acells which respond to nerve growth factor. Greene LA,Tischler AS. Proceedings of the National Academy of Sciences of the United States of America . 1976
[5]  
PC12pheochrom ocytom a cells: culture, nerve growth factortreatm entand experim entalexploitation. Greene LA,Aletta JM,Rukenstein A,et al. Methods in Enzymology . 1987
[6]  
The quantitative bioassay ofnerve growth factor: use of frozen prim ed’PC1pheochrom ocytom a cells. Rukenstein A,Greene LA. Brain Research . 1983