肉苁蓉苷对C17.2神经干细胞增殖的影响

被引：8

作者：

覃威

魏维

许茜

蔡晶

机构：

[1] 福建中医药大学中西医结合学院

来源：

海峡药学 | 2015年 / 27卷 / 12期

关键词：

肉苁蓉苷; 神经干细胞; 增殖;

D O I：

暂无

中图分类号：

R329 [人体组织学];

学科分类号：

100101 ;

摘要：

目的观察肉苁蓉苷对C17.2神经干细胞增殖的影响,并探讨其可能的作用机制。方法体外常规培养C17.2神经干细胞,按1∶3传代。传代培养的神经干细胞C17.2,分为给药组0.0625mg·m L-1、0.125mg·m L-1、0.25mg·m L-1和空白组,培养24h。镜下观察C17.2神经干细胞的形态,噻唑蓝(MTT)法和Brdu免疫标记检测细胞的活性及增殖能力。结果 MTT检测结果显示,药物干预后,各组神经干细胞的活性均有增加,与空白组比较,差异有统计学意义(P<0.05);Brdu免疫标记显示,与对照组相比,高中剂量组的Brdu阳性细胞数显著增多。结论肉苁蓉苷可在一定程度上促进神经干细胞的增殖,可能成为神经康复的潜在药物。

引用

页码：263 / 265+296 +296

页数：4

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