siRNA阻断NF-κB信号通路对食管鳞癌细胞增殖、耐药的影响

被引：8

作者：

田芳 ^{[1
]}

田卫红 ^{[2
]}

许培荣 ^{[3
]}

刘红涛 ^{[3
]}

薛乐勋 ^{[3
]}

机构：

[1] 郑州大学基础医学院病理生理学教研室

[2] 安阳市肿瘤医院妇瘤科

[3] 郑州大学细胞生物学研究室

来源：

郑州大学学报(医学版) | 2007年 / 01期

关键词：

食管肿瘤; NF-kappaB; RNA干扰; siRNA;

D O I：

10.13705/j.issn.1671-6825.2007.01.016

中图分类号：

R735.1 [食管肿瘤];

学科分类号：

100214 ;

摘要：

目的:通过RNA干扰(RNAi)阻断食管鳞癌细胞中NF-κB信号通路,研究其与肿瘤细胞增殖、耐药的关系。方法:使用NF-κB p65 siRNA分别转染人食管鳞癌Eca109和EC9706细胞72h,以未转染的Eca109和EC9706细胞为对照,采用Western blot检测p65蛋白的表达;MTT法检测转染NF-κBp65siRNA24h、48h、72h后Eca109和EC9706细胞的增殖情况及转染同时联合应用不同质量浓度5-Fu(0mg/L,16.35mg/L,32.7mg/L,327mg/L,3270mg/L,6540mg/L)对Eca109和EC9706细胞增殖的影响。结果:①NF-κB亚单位p65在Eca109和EC9706细胞质中高表达,p65siRNA可有效阻断p65蛋白表达。②MTT实验表明,转染组细胞存活率较未转染组明显下降(P<0.05);与化疗药5-Fu联用,转染组和未转染组细胞的增殖活性随着5-Fu浓度的增加有下降趋势,但在同一浓度,转染p65siRNA的细胞与未转染组相比,细胞增殖活性明显下降(P<0.05)。结论:应用RNAi技术可有效干扰p65的表达,抑制食管鳞癌细胞的增殖,增强对化疗药5-Fu的敏感性。因此,可将阻断NF-κB信号通路作为基因治疗的靶点。

引用

收藏

页码：41 / 44

页数：4

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