丹参诱导大鼠骨髓间充质干细胞向神经样细胞分化

被引：14

作者：

王勇 ^{[1
]}

陆长青 ^{[2
]}

王凡 ^{[2
]}

机构：

[1] 大理学院基础医学院人体解剖教研室

[2] 四川大学华西基础与法医学院人体解剖学教研室

来源：

解剖学杂志 | 2007年 / 02期

关键词：

骨髓间充质干细胞; 神经样细胞; 丹参; 诱导分化; 大鼠;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理];

学科分类号：

1008 ;

摘要：

目的:探讨骨髓间充质干细胞(marrow stromal stem cells,MSCs)向神经细胞分化中神经蛋白分子及相关基因的表达情况。方法:分离提取大鼠的MSCs,体外培养扩增。用含丹参的无血清的L-DMEM培养基进行诱导,并以不含丹参的无血清L-DMEM培养基作为对照。提取两组的MSCs总RNA,RT-PCR检测ngn-1、mash-1的表达。结果:丹参诱导90 min后,细胞发生了明显的形态学变化,大多数细胞转变为类似双极或多极神经元样形态,伸出轴突或树突样突起。免疫细胞化学显示诱导后的MSCs表现为nestin、NSE、GFAP染色阳性,对照组为阴性。未经诱导的MSCs ngn-1,mash-1 mRNA为阴性,诱导后有表达。结论:丹参可诱导大鼠MSCs向神经前体细胞和神经细胞分化。MSCs向神经元样的分化可能与ngn-1,mash-1有关。

引用

页码：207 / 210

页数：4

共 10 条

[1]

Sciatic nerve regenera- tion in rats induces by transplantation of in vitro differentiated- bone-marrow stromal cells. Dezawa M,Takahashi L,Esaki M,et al. European Journal of Neuroscience . 2001

[2]

Expression of GFAP (glial fibrillary acidic protein) antigenicity and differentiation of glioma tumuor cells of astrocytic origin. Kaluza J,Adamek D. Folia Histochemica et Cytochemica . 1990

[3]

Developmental Biology[C]. 2008年国际口腔及颅颌前沿研究研讨会,1600

[4]

Neurogenin promotes neurogenesis and inhibits glial differentiation by independent mechanisms. Sun Y,Nadal-Vicens M,Misono S,et al. Cell . 2001

[5]

Enteric dopaninergic neurons: definition,developmental lineage,and effects of extrinsic dener- vation. Li ZS,Pham TD,Tamir H,et al. The Journal of Neuroscience . 2004

[6]

Turning blood into brain:cells bearing neuronal antigens generated in vivo from bone marrow. Mezey E,Chandross KJ.Harta G,et al. Science . 2000

[7]

Marrow stromal cells mi- grate throughout forebrain and cerebellum and they differentiate into astrocytes after injection intoneonatal mouse brains. Kopen GC,Prockop DJ,Phinney DG. Proceedings of the National Academy of Sciences of the United States of America . 1999

[8]

Electron mi- croscopic localization of neuron-specific enolase in rat and mouse brain. Vinores SA,Herman MM,Rubinstein LJ,et al. Journal of Histochemistry and Cytochemistry . 1984

[9]

From marrow to brain:expression of neuronal phenotypes in adult mice. Brazelton TR,Rossi FMV,Keshet GI,et al. Science . 2000

[10]

Adult bone marrow stromal cells differentiate into neurons. Sanchez-Ramos J,Song S,Cardczc-Pelaez F,et al. Journal of Neuroscience Research . 2000

← 1 →