非调制式荧光仪测定叶绿素荧光参数的研究

被引：14

作者：

赵弢 ^{[1
]}

高志奎 ^{[1
]}

徐广辉 ^{[1
]}

王梅 ^{[1
]}

高荣孚 ^{[2
]}

机构：

[1] 河北农业大学

[2] 北京林业大学

来源：

生物物理学报 | 2006年 / 01期

基金：

国家科技攻关计划;

关键词：

非调制式荧光仪; 调制式荧光仪; 叶绿素荧光动力学;

D O I：

暂无

中图分类号：

Q94-3 [植物学研究和植物学实验];

学科分类号：

摘要：

为了利用非调制式荧光仪获得调制式荧光仪测定的叶绿素荧光动力学参数,采用植物效率(PEA)仪进行了叶绿素荧光参数测定程式的4要素(光化光强度及其照射时间、饱和激发光强度及其照射时间)以及节约测定时间和仪器内存的测定技术研究。结果表明,暗适应10min后,在设定180μmol·m-2·s-1光化光照射360s时间内,以连续2次1950μmol·m-2·s-1饱和激发光照射3s的测定程式,在7种植物的叶绿素荧光参数的测定中获得了较为满意的测定结果,而且与Strasser等测定程式相比,1次测定需时从600s缩短为360s、耗用仪器内存从76.9"减少为2.56"。

引用

页码：34 / 38

页数：5

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