高效液相色谱-串联质谱法对猪肉中四种大环内酯类药物残留量的测定

被引：6

作者：

夏曦 ^{[1
,2
]}

李晓薇 ^{[1
,2
]}

江海洋 ^{[1
,2
]}

李建成 ^{[1
,2
]}

安保超 ^{[1
]}

丁双阳 ^{[1
,2
]}

机构：

[1] 中国农业大学动物医学院

[2] 国家兽药残留基准实验室

来源：

分析测试学报 | 2008年 / S1期

关键词：

high performance liquid chromatography-tandem mass spectrometry; pork; macrolide;

D O I：

暂无

中图分类号：

R155.5 [食品卫生与检验];

学科分类号：

100403 ;

摘要：

A simple and sensitive method was developed for the simultaneous determination of four macrolides in pork using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Homogenized sample was extracted with NaH2PO4 buffer and acetonitrile solution,and defatted with n-hexane.Further cleanup was performed on a Sep-Pakt C18 solid-phase extraction cartridge.The compounds were determined by LC-MS/MS operated in positive electrospray ionization mode.The limits of detection(LODs) were 0.5 μg/kg.The average recoveries at three spiked concentration levels of 1.0,5.0,10.0 μg/kg were in the range of 70%-110%,with the intra-day RSD of less than 12.9% and inter-day RSD of less than 13.4%.

引用

页码：224 / 226+230 +230

页数：4