心肌细胞横管的形态发生:Junctophilin-2的作用

被引：6

作者：

韩晶

吴昊迪

王其伟

王世强

机构：

[1] 北京大学生命科学学院,生物膜与膜工程国家重点实验室

来源：

中国科学:生命科学 | 2013年 / 43卷 / 09期

关键词：

横管; Junctophilin-2; 心肌细胞; 形态发生;

D O I：

暂无

中图分类号：

Q954.48 [组织器官和系统发生（形体变化）];

学科分类号：

071002 [动物学];

摘要：

横管系统是心脏和肌肉细胞兴奋收缩偶联的结构基础.在新生鼠的心肌细胞中并不存在横管结构,横管的形态发生是心肌细胞成熟过程中的一个重要标志.本文对心肌细胞成熟过程中横管的形态变化进行定量分析,并对连接横管和肌质网的关键分子Junctophilin-2(JP2)在这个过程中所发挥的作用进行研究.对心肌细胞图像的分析显示,在横管成熟的过程中,横管的横向组分逐渐增加,纵向组分逐渐减少.通过对横管图像进行傅里叶变换分析,发现横管排列的规律性随心肌细胞的成熟而逐渐增加.横管的这些变化伴随着JP2蛋白表达量的上升,并且可以被JP2表达量的敲减所逆转.这些现象表明,JP2在横管形态发生过程中发挥重要作用.

引用

页码：738 / 744

页数：7

共 16 条

[1]

Junctophilin 2 knockdown interfere with mitochondrium status in ESC-CMs and cardiogenesis of ES cells [J].

Liang, Xingguang ;

Mei, Yuqin ;

Huang, Xin ;

Shen, Guofang ;

Zhu, Danyan ;

Yu, Yongping ;

Wang, Jianan ;

Lou, Yijia .

JOURNAL OF CELLULAR BIOCHEMISTRY, 2012, 113 (09) :2884-2894

[2]

Disrupted Junctional Membrane Complexes and Hyperactive Ryanodine Receptors After Acute Junctophilin Knockdown in Mice [J].

van Oort, Ralph J. ;

Garbino, Alejandro ;

Wang, Wei ;

Dixit, Sayali S. ;

Landstrom, Andrew P. ;

Gaur, Namit ;

De Almeida, Angela C. ;

Skapura, Darlene G. ;

Rudy, Yoram ;

Burns, Alan R. ;

Ackerman, Michael J. ;

Wehrens, Xander H. T. .

CIRCULATION, 2011, 123 (09) :979-U123

[3]

T-Tubule Remodeling During Transition From Hypertrophy to Heart Failure [J].

Wei, Sheng ;

Guo, Ang ;

Chen, Biyi ;

Kutschke, William ;

Xie, Yu-Ping ;

Zimmerman, Kathy ;

Weiss, Robert M. ;

Anderson, Mark E. ;

Cheng, Heping ;

Song, Long-Sheng .

CIRCULATION RESEARCH, 2010, 107 (04) :520-U163

[4]

Ca2+ sparks and cellular distribution of ryanodine receptors in developing cardiomyocytes from rat [J].

Snopko, Rose M. ;

Ramos-Franco, Josefina ;

Di Maio, Alessandro ;

Karko, Kimberly L. ;

Manley, Christopher ;

Piedras-Renteria, Erika ;

Mejia-Alvarez, Rafael .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2008, 44 (06) :1032-1044

[5]

T-tubule formation in cardiacmyocytes: two possible mechanisms? [J].

Alessandro Di Maio ;

Kimberly Karko ;

Rose M. Snopko ;

Rafael Mejía-Alvarez ;

Clara Franzini-Armstrong .

Journal of Muscle Research and Cell Motility, 2007, 28 :231-241

[6]

Uncoupling store-operated Ca2+ entry and altered Ca2+ release from sarcoplasmic reticulum through silencing of junctophilin genes [J].

Hirata, Yutaka ;

Brotto, Marco ;

Weisleder, Noah ;

Chu, Yi ;

Lin, Peihui ;

Zhao, Xiaoli ;

Thornton, Angela ;

Komazaki, Shinji ;

Takeshima, Hiroshi ;

Ma, Jianjie ;

Pan, Zui .

BIOPHYSICAL JOURNAL, 2006, 90 (12) :4418-4427

[7]

Calcium Biology of the Transverse Tubules in Heart.[J].LONG‐SHENGSONG;SILVIAGUATIMOSIM;LETICIAGóMEZ‐VIQUEZ;ERIC A.SOBIE;ANDREWZIMAN;HALIHARTMANN;W. J.LEDERER.Annals of the New York Academy of Sciences.2006, 1

[8]

Temperature dependence and thermodynamic properties of Ca2+ sparks in rat cardiomyocytes [J].