SSR标记用于玉米自交系遗传变异与优势类群划分的研究(英文)

被引:32
作者
刘杰
刘公社
朱至清
陈刚
机构
[1] 中国科学院植物研究所
[2] 北京市农林科学院
关键词
玉米自交系; SSR; 杂种优势群;
D O I
暂无
中图分类号
S513 [玉米(玉蜀黍)];
学科分类号
摘要
采用 SSR和杂种优势聚类方法分析我国 1 5个玉米骨干自交系的遗传变异 ,并初步进行了杂种优势类群划分。从 62个 SSR引物中筛选出的 40对有效引物对 1 5个玉米自交系进行了分析 ,共检测到 1 88个等位基因变异 ,每个 SSR座位的等位基因数 2~ 9个 ,平均为 4.7个。 SSR位点的多态信息含量 PIC值平均为 0 .675 ,分布范围在 0 .360~ 0 .85 1之间。根据SSR数据对供试材料进行遗传相似性分析 ,Nei氏相似性系数分布在 0 .5 74~ 0 .777之间。 1 0对多态性高的 SSR引物可有效区分 1 5个自交系。应用 SSR聚类分析的结果与系谱关系相一致 ,与杂种优势聚类法相比较 ,SSR方法具有效率高、结果可靠、可标准化的特点。对 SSR方法在玉米育种实践上的应用进行了初步探讨
引用
收藏
页码:17 / 26
页数:10
相关论文
共 9 条
[1]   几个常用玉米自交系的优势类群分析 [J].
池书敏,刘志增 .
河北农业大学学报, 1995, (01) :22-26
[2]  
The use of microsatellite markers for detection of genetic diversity in barley populations[J] . D. Struss,J. Plieske.TAG Theoretical and Applied Genetics . 1998 (1-2)
[3]  
Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers[J] . P. Ajmone Marsan,P. Castiglioni,F. Fusari,M. Kuiper,M. Motto.TAG Theoretical and Applied Genetics . 1998 (2)
[4]  
An evaluation of the utility of SSR loci as molecular markers in maize (Zea mays L.): comparisons with data from RFLPS and pedigree[J] . J. S. C. Smith,E. C. L. Chin,H. Shu,O. S. Smith,S. J. Wall,M. L. Senior,S. E. Mitchell,S. Kresovich,J. Ziegle.TAG Theoretical and Applied Genetics . 1997 (1-2)
[5]  
Identification of polymorphic, conserved simple sequence repeats (SSRs) in cultivated Brassica species[J] . A. K. Szewc-McFadden,S. Kresovich,S. M. Bliek,S. E. Mitchell,J. R. McFerson.Theoretical and Applied Genetics . 1996 (4)
[6]  
Assessment of genetic diversity in dent and popcorn ( Zea mays L.) inbred lines using inter-simple sequence repeat (ISSR) amplification[J] . Ramesh V. Kantety,Xiaoping Zeng,Jeffrey L. Bennetzen,Brent E. Zehr.Molecular Breeding . 1995 (4)
[7]  
Use of single-primer DNA amplifications in genetic studies of peanut ( Arachis hypogaea L.)[J] . Tracy Halward,Tom Stalker,Elizabeth LaRue,Gary Kochert.Plant Molecular Biology . 1992 (2)
[8]  
Genetic diversity for restriction fragment length polymorphisms and heterosis for two diallel sets of maize inbreds[J] . A. E. Melchinger,M. Lee,K. R. Lamkey,A. R. Hallauer,W. L. Woodman.Theoretical and Applied Genetics . 1990 (4)
[9]  
Heterosis in relation to genetic divergence and specific combining ability in groundnut ( Arachis hypogaea L.)[J] . V. Arunachalam,A. Bandyopadhyay,S. N. Nigam,R. W. Gibbons.Euphytica . 1984 (1)