基于石墨烯-海藻酸钠复合材料的辣根过氧化物酶传感器研究

被引：1

作者：

肖福兵 ^{[1
]}

杨欣 ^{[1
,2
]}

田锐 ^{[1
]}

张瑞莲 ^{[1
]}

梅琼之 ^{[1
]}

吴朝阳 ^{[1
]}

机构：

[1] 湖南大学化学化工学院,化学生物传感与计量学国家重点实验室

[2] 怀化学院化学与化学工程系

来源：

化学传感器 | 2014年 / 34卷 / 01期

关键词：

海藻酸钠; 石墨烯; 辣根过氧化物酶;

D O I：

暂无

中图分类号：

TP212 [发送器（变换器）、传感器];

学科分类号：

080202 ;

摘要：

构建了一种以海藻酸钠-石墨烯(SA-GR)为基底的新型过氧化氢电化学酶传感器。利用滴涂法将生物相容性良好的海藻酸钠-石墨烯复合物固定在玻碳电极表面,再通过酰胺键将HRP连接在SA-GR复合膜上,从而制备出了性能良好的过氧化氢电化学酶传感器。该传感器重现性好、灵敏度较高,并且响应速度快(3 s),米氏常数较低(Km=0.663),对H2O2检测的线性范围为1.0×10-4～1.2×10-3mol/L,检测下限为5.7×10-6mol/L。

引用

页码：57 / 63

页数：7

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