纳米银颗粒增强聚合酶链式反应长片段DNA反复扩增的特异性

被引：6

作者：

王群

李静

曹小红

张治洲

机构：

[1] 泰达BIO-X系统生物技术研究中心天津科技大学食品工程与生物技术学院

来源：

天津科技大学学报 | 2007年 / 02期

关键词：

聚合酶链式反应; 长片段; 纳米银; 特异性; 反复扩增;

D O I：

10.13364/j.issn.1672-6510.2007.02.001

中图分类号：

Q503 [生物化学技术];

学科分类号：

摘要：

基因操作者经常需要把数量稀少的DNA样品进行反复扩增以供进一步研究之用.在反复扩增过程中,上一次扩增产物当作下一次扩增的模板使用.对于较短的DNA片段(几百个碱基),一般反复扩增不会遇到特别的困难;但是较长DNA片段(几千到几万个碱基)的反复扩增一般会极大地降低聚合酶链式反应(PCR)的特异性.实验中发现,当扩增较长片段基因时,在常规PCR热循环体系中添加粒径平均为70 nm的银颗粒,使反应体系纳米银颗粒浓度达到0.1～1.2μg/μL,可以在一定程度上保持PCR反复扩增反应的特异性.其机理之一可能是常规PCR反应体系在添加了纳米银颗粒后改善了溶液的导热性能.

引用

页码：1 / 5

页数：5

共 16 条

[1] Positioning isolation and biochemical analysis of single DNA molecules based on nano-manipulation and single-molecule PCR. Lu J,Li H,An H,et al. Journal of the American Chemical Society . 2004
[2] Primer-directed enzymatic amplification of DNA with a thermostable DNA-ploymerase. Saiki R K,Gelfand D H,Stoffel S,et al. Science . 1988
[3] Thermal transport in nanofluids. Eastman J A,Phillpot S R,Choi S U S,et al. Annual Review of Materials Research . 2004
[4] Enhancing the efficiency of a PCR using gold nanoparticles. Li M,Lin Y,Wu C C,et al. Nucleic Acids Research . 2005
[5] Colorimetric detection of DNA sequences based on electrostatic interactions with unmodified gold nanoparticles. Li H,Rothberg L. Proceedings of the National Academy of Sciences of the United States of America . 2004
[6] Thermal conductivity of nanoparticle suspensions. Putnam S A,Cahill D G,Braun P V,et al. Journal of Applied Physics . 2006
[7] Effect of nanoparticles on critical heat flux of water in pool boiling heat transfer. You S M,Kim J H,Kim K H. Applied Physics Letters . 2003
[8] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Mullis K B,Faloona F A. Methods in Enzymology . 1987
[9] Measuring thermal conductivity of fluids containing oxide nanoparticles. Lee S,Choi U SL,i S,et al. Journal of Heat Transfer . 1999
[10] Anomalously increased effective thermal conductivities of ethylene glycol-based nanofluids containing copper nanoparticles. Eastman J A,Choi S U S,Li Se,t al. Applied Physics . 2001

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