内皮素对大鼠肺动脉平滑肌细胞膜钾通道的影响

被引：2

作者：

樊再雯

张珍祥

徐永健

机构：

[1] 华中科技大学同济医学院附属同济医院卫生部呼吸系疾病重点实验室

[2] 华中科技大学同济医学院附属同济医院卫生部呼吸系疾病重点实验室湖北武汉

[3] 湖北武汉

来源：

药学学报 | 2004年 / 01期

关键词：

钾通道; 内皮素; 膜片钳; 肺动脉平滑肌细胞;

D O I：

10.16438/j.0513-4870.2004.01.003

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

目的　观察内皮素 (ET 1)对大鼠肺动脉平滑肌细胞 (PASMCs)膜电压门控钾通道 (KV)的作用。方法　用全细胞膜片钳记录方法研究ET 1对膜电位 (Em)、膜电容 (Cm)、电压门控钾电流 (IKV)的影响。结果　ET 1可引起PASMCs去极化 ,并抑制IKV,抑制IKV 时间明显早于其对Em 变化的影响 ,ET 1对IKV 的影响呈可逆性和浓度依赖性 ,在有钙的环境中 ,ET 1对IKV 峰电流的抑制率明显高于无钙时。结论　ET 1可浓度依赖性的抑制IKV ,使常氧大鼠PASMCs去极化 ,且ET 1对IKV 的抑制作用早于对Em 的影响 ,这些作用不完全依赖于钙的参与 ,但钙可加强ET 1对IKV 的抑制作用。

引用

页码：9 / 12

页数：4

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