利用拟杆菌分子标记物对粪便污染溯源的研究进展

被引：11

作者：

张曦 ^{[1
,2
]}

朱昌雄 ^{[2
]}

朱红惠 ^{[1
]}

机构：

[1] 广东省微生物研究所广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室

[2] 中国农业科学院农业环境与可持续发展研究所

来源：

微生物学报 | 2011年 / 51卷 / 07期

关键词：

拟杆菌; 宿主特异性; 生物标记; 微生物溯源;

D O I：

10.13343/j.cnki.wsxb.2011.07.001

中图分类号：

X502 [污染分析与测定];

学科分类号：

071012 ; 0713 ; 083002 ;

摘要：

拟杆菌(Bacteroidales)是粪便污染的主要标志之一,近年来被广泛应用在水体微生物溯源领域,并展现了良好的应用前景。拟杆菌微生物溯源的优势在于不需要纯培养过程,直接根据拟杆菌具有的宿主特异性生物标记引物设计,通过检测水体中拟杆菌生物标记的存在情况来判断粪便污染来源。本文从人、猪、反刍动物等拟杆菌特异性生物标记的发现、引物设计以及其应用综述了拟杆菌在微生物溯源中的研究进展,指出了应用过程中的局限性。本文还对拟杆菌在溯源中的应用进行了展望。提出了寻找新的宿主特异性拟杆菌生物标记是研究的主要方向,在应用研究中应注重与其他溯源方法相结合以使溯源结果更加准确。

引用

页码：863 / 868

页数：6

共 10 条

[1] 拟杆菌的研究及应用 [J].

吴彦彬 ;

李亚丹 ;

李小俊 ;

边艳青 .

生物技术通报, 2007, (01) :66-69

[2] Specificity and sensitivity evaluation of novel and existing Bacteroidales and Bifidobacteria-specific PCR assays on feces and sewage samples and their application for microbial source tracking in Ireland [J].

Dorai-Raj, Siobhan ;

O'Grady, Justin ;

Colleran, Emer .

WATER RESEARCH, 2009, 43 (19) :4980-4988

[3]

Molecular identification of fecal pollution sources in water supplies by host-specific fecal DNA markers and Terminal Restriction Fragment Length Polymorphism profiles of 16S rRNA gene[J] . Ju-Yong Jeong,Kyung-Ik Gil,Kyong-Hee Lee,Jong-Ok Ka.The Journal of Microbiology . 2008 (6)

[4]

Sourcing faecal pollution: A combination of library-dependent and library-independent methods to identify human faecal pollution in non-sewered catchments[J] . W. Ahmed,J. Stewart,T. Gardner,D. Powell,P. Brooks,D. Sullivan,N. Tindale.Water Research . 2007 (16)

[5]

16S rRNA-based assays for quantitative detection of universal, human-, cow-, and dog-specific fecal Bacteroidales : A Bayesian approach[J] . Beverly J. Kildare,Christian M. Leutenegger,Belinda S. McSwain,Dustin G. Bambic,Veronica B. Rajal,Stefan Wuertz.Water Research . 2007 (16)

[6] Microbial source tracking in a small southern California urban watershed indicates wild animals and growth as the source of fecal bacteria [J].

Jiang, Sunny C. ;

Chu, Weiping ;

Olson, Betty H. ;

He, Jian-Wen ;

Choi, Samuel ;

Zhang, Jenny ;

Le, Joanne Y. ;

Gedalanga, Phillip B. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2007, 76 (04) :927-934

[7] Quantification of host-specific Bacteroides–Prevotella 16S rRNA genetic markers for assessment of fecal pollution in freshwater [J].

Satoshi Okabe ;

Noriko Okayama ;

Olga Savichtcheva ;

Tsukasa Ito .

Applied Microbiology and Biotechnology, 2007, 74 :890-901

[8]

A dynamic partnership: Celebrating our gut flora[J] . Cynthia L. Sears.Anaerobe . 2005 (5)

[9]

Source tracking fecal bacteria in water: a critical review of current methods[J] . Cynthia L. Meays,Klaas Broersma,Rick Nordin,Asit Mazumder.Journal of Environmental Management . 2004 (1)

[10] Development of a membrane-array method for the detection of human intestinal bacteria in fecal samples [J].

Wang, RF ;

Kim, SJ ;

Robertson, LH ;

Cerniglia, CE .

MOLECULAR AND CELLULAR PROBES, 2002, 16 (05) :341-350

← 1 →