外源H2O2对湖北海棠根系线粒体膜透性和细胞核DNA的影响

被引:11
作者
马怀宇
杨洪强
机构
[1] 山东农业大学园艺学院
基金
高等学校博士学科点专项科研基金;
关键词
根系; 线粒体膜通透性; 线粒体膜电位; 细胞程序性死亡; H2O2; 湖北海棠;
D O I
暂无
中图分类号
S661.4 [海棠类]; Q945.78 [协迫生理学];
学科分类号
090201 ; 0903 ;
摘要
用0.024%(V/V)H2O2处理湖北海棠(MalushupehensisRehd.)实生苗根系,30min后,根系线粒体膜通透性明显增大,线粒体膜电位(?ψm)和线粒体内Cytc/a吸光度比值下降;60min后,根系细胞核DNA发生片段化降解。经荧光染料吖啶橙染色后,可见H2O2处理60min以上的根系压片中出现了清晰致密的黄绿色荧光斑,呈现出细胞程序性死亡(PCD)的特征。
引用
收藏
页码:551 / 556
页数:6
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