从线粒体控制区全序列变异看短颌鲚和湖鲚的物种有效性

被引：88

作者：

唐文乔

胡雪莲

杨金权

机构：

[1] 上海水产大学省部共建种质资源发掘与利用教育部重点实验室

来源：

生物多样性 | 2007年 / 03期

关键词：

鲚属; 物种有效性; 线粒体控制区; 分子系统发育;

D O I：

暂无

中图分类号：

Q951 [动物演化与发展];

学科分类号：

摘要：

经克隆测序获得我国七丝鲚(Coiliagrayii)、凤鲚(C.mystus)、刀鲚(C.nasus)和短颌鲚(C.brachygnathus)以及太湖湖鲚(C.nasus taihuensis)等4个种和1亚种32尾个体的mtDNAD-loop区全序列,以日本鳀(Engraulis japonicus)和秘鲁鳀(E.ringens)为外类群构建了中国鲚属的分子系统发育树,并讨论了短颌鲚和湖鲚的物种有效性。结果显示,七丝鲚的D-loop区全序列长1,208bp,凤鲚1,279-1,361bp,刀鲚1,252-1,290bp,短颌鲚1,214-1,252bp,湖鲚1,252-1,442bp,除七丝鲚外的其他种类个体间均表现出序列长度的多态性。短颌鲚、刀鲚和湖鲚三者间的平均K2-P遗传距离仅为0.011-0.020,明显小于它们与凤鲚、七丝鲚及外类群间的遗传距离(0.051-0.349)。以邻接法和最大简约法构建的系统发育树表明,刀鲚、短颌鲚及湖鲚均未各自构成单系,而是共同构成一个单系群,三者并未发生显著分化。研究表明,短颌鲚和湖鲚为刀鲚的淡水生态型种群,并非有效物种。系统发育分析表明,中国鲚属3个有效物种间以凤鲚最为原始,刀鲚和七丝鲚为姐妹群,处于较进化的位置。推测凤鲚可能是鲚属祖先种最早从起源中心扩散到西北太平洋的后裔,而刀鲚和七丝鲚则是凤鲚在演化过程中分别适应寒冷和温暖气候而分化出的物种。

引用

页码：224 / 231

页数：8

共 19 条

[1]

Nei M(2004)MEGA3:Integrated soft-ware for Molecular Evolutionary Genetics Analysis and sequence alignment. Kumar S,Tamura K. Briefings in Bioinformatics .

[2]

Brun G(2001)Partial combination applied to phylogeny of European cyprinids using the mitochondrial control re-gion. Gilles A,Lecointre G,Miquelis A,Loerstcher M,Chappaz R. Molecular Phylogenetics and Evolution .

[3]

Hig-gins DG(1997)The Clustal_X windows interface:flexible strategies ed for multiple sequences alignment aided by quality analysis tools. Thompson JD,Gibson TJ,Plewwniak F,Jeanmougin F. Nucleic Acids Research .

[4]

Ma L(2005)Morphological differences between close populations discernible by multivariateanalysis:a case study of genus Coilia(Teleostei:Clupei-forms). Cheng QQ,Lu DR. Aquatic Living Resources .

[5]

Brown GC(1988)Length and restric-tion site heteroplasmy in the mitochondrial DNA of American shad(Alosa sapidissima). Bentzen P,Leggett WC. Genetics .

[6]

Teng H(2002)Sequence variations in the mitochondrial DNA control region and their implications for the phylogeny of the Cypriniformes. Liu HZ,Tzeng C. Canadian Journal of Zoology .

[7]

Crandall KA(1998)MODELTEST:testing the model of DNA substitution. Posada D. Bioinformatics .

[8]

Moritz C(1994)Sequence evolution and phylogenetic signal in control region and cytochrome b sequences of rainbow fishes(Melanotaenii-dae). Zhu D,Jamieson BG,Hugall A. Molecular Biology .

[9]

Gautier C(1996)SEAVIEW and PHYLO_WIN:two graphic tools for sequence alignment and molecular phylogeny. Galtier N,Gouy M. Computer Applications in the Biosciences .

[10]

Sacone C(1997)Mammalian mitochondrial D-loop region structure analy-sis:identification of new conserved sequences and their functional and evolutionary implifications. SbisàE,Tanzariello F,Reyes A,Pesole G. Gene .

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