阿魏酸钠对缺氧致脑血管内皮细胞NF-κB、IκBα表达的影响

被引：12

作者：

陈莉芬 ^{[1
]}

陶陶 ^{[2
]}

余震 ^{[1
]}

胡长林 ^{[1
]}

机构：

[1] 重庆医科大学第二附属医院神经内科

[2] 贵阳医学院附属医院神经内科

来源：

第三军医大学学报 | 2007年 / 03期

关键词：

阿魏酸钠; 脑血管内皮细胞; 缺氧; NF-κB; IκBα;

D O I：

10.16016/j.1000-5404.2007.03.018

中图分类号：

R743 [脑血管疾病];

学科分类号：

摘要：

目的研究阿魏酸钠对缺氧脑血管内皮细胞核转录因子κB(nuclearfactor-κB,NF-κB)及IκBα表达的影响。方法建立大鼠脑皮质微血管内皮细胞(brainmicrovascularendothelialcell,BMEC)体外培养模型,并施加12h缺氧条件,分为对照组、缺氧组、阿魏酸钠组。应用MTT法测定细胞活性,采用放射免疫方法测定内皮素(endothelin-1,ET-1)含量,采用免疫细胞化学法检测BMEC的NF-κB及IκBα表达。结果阿魏酸钠(100μg/ml)能抑制缺氧诱导的ET-1释放(P<0.01)。缺氧刺激血管内皮细胞NF-κB的表达,降低IκBα的表达,阿魏酸钠可以显著抑制缺氧内皮细胞中NF-κB的表达及核移位现象,增加IκBα的表达和BMEC活性。结论阿魏酸钠对大鼠脑皮质BMEC缺氧损伤具有保护作用,机制与其减少BMEC分泌ET-1,抑制NF-κB蛋白表达,促进IκBα蛋白表达有关。

引用

页码：228 / 230

页数：3

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