HPLC-DAD-Q-TOF-MS/MS法的银黄颗粒主要成分定性与定量研究

被引：57

作者：

向青 ^{[1
,2
]}

王小花 ^{[2
]}

林慧 ^{[2
]}

林婧 ^{[3
]}

许文 ^{[1
,2
,3
]}

机构：

[1] 福建中医药大学生物医药研发中心

[2] 福建中医药大学药学院

[3] 福建中医药大学中西医结合研究院

来源：

中成药 | 2015年 / 37卷 / 01期

关键词：

银黄颗粒; 高效液相色谱串联四级杆飞行时间质谱; HPLC-DAD; 化学成分;

D O I：

暂无

中图分类号：

R286 [中药品]; O657.63 [质谱分析];

学科分类号：

070302 [分析化学]; 100403 [营养与食品卫生学];

摘要：

目的采用高效液相色谱串联四极杆飞行时间质谱（HPLC-Q-TOF-MS/MS）快速鉴定银黄颗粒化学成分和HPLC-DAD法同时定量测定银黄颗粒中15种成分（獐牙菜苷、马钱苷、新绿原酸、绿原酸、隐绿原酸、咖啡酸、芦丁、异绿原酸B、异绿原酸A、异绿原酸C、野黄芩苷、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素）。方法分析采用月旭Ultimate-XB C18色谱柱（4.6 mm×150 mm,5μm）,流动相为乙腈-0.1%甲酸水,梯度洗脱,体积流量0.8 m L/min,柱温30℃。飞行时间质谱,采用负离子模式扫描。二极管阵列检测器的检测波长为240、276、326 nm。结果 HPLC-Q-TOF-MS/MS法推断鉴定银黄颗粒中的44种成分,HPLC-DAD定量分析银黄颗粒中15种成分在考察的质量浓度范围内,与峰面积之间呈良好的线性关系（r>0.999 0）;回收率均在97.3%100.9%范围内,RSD为1.4%2.8%。结论通过HPLC-Q-TOF-MS/MS法和HPLC-DAD法鉴定银黄颗粒的化学成分优于HPLC法,尤其测定环烯醚萜苷类成分（獐牙菜苷、马钱苷）可为综合评价银黄制剂的质量提供参考。

引用

页码：105 / 112

页数：8

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