幽门螺杆菌对体外培养的胃上皮细胞增殖与凋亡的影响

被引：9

作者：

杨艺

邓长生

彭俊忠

机构：

[1] 武汉大学中南医院消化内科

[2] 武汉科技大学医学院

来源：

中华微生物学和免疫学杂志 | 2002年 / 01期

关键词：

幽门螺杆菌; 细胞增殖; 细胞凋亡; Ki-67抗原; Hoechst33258荧光染色; 流式细胞术;

D O I：

暂无

中图分类号：

R735.2 [胃肿瘤];

学科分类号：

100214 ;

摘要：

目的　研究H .pylori对体外培养的胃上皮细胞增殖与凋亡的影响。方法　以SGC 790 1细胞作为H .pylori感染的体外细胞模型 ,用Ki 6 7抗原的免疫组化分析检测了H .pylori标准菌株NCTC 116 37活菌对胃上皮细胞增殖的影响 ,同时用流式细胞术、荧光染色技术检测了细胞凋亡率。结果　H .pylori在较低浓度 (≤ 1.6× 10 5CFU/ml)时对细胞增殖有促进作用 ,而在较高浓度 (≥ 8× 10 5CFU/ml)时抑制细胞增殖。H .pylori以浓度依赖方式诱导胃上皮细胞凋亡 ,Hoechst 332 5 8荧光染色和流式细胞术两种方法所得结果一致。结论　细胞凋亡与增殖间的不平衡亦可部分解释人体感染H .pylori后所表现的多样化结局

引用

页码：14 / 17

页数：4

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