提高人重组IL-6在真核细胞中表达的新途径

被引：3

作者：

刘红涛

袁勇

张颖妹

王应

狄春辉

马大龙

机构：

[1] 北京医科大学免疫系!北京

来源：

中国肿瘤生物治疗杂志 | 1996年 / 02期

关键词：

白细胞介素6; 基因转染; 内含子; COS-7细胞;

D O I：

暂无

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

为提高IL-6在真核系统中的表达效率,我们在构建真核表达载体pcDNA3IL-6的基础上,用表达载体pCI中的CMV启动子和下游所带的β-球蛋白的第一个内含子,置换了pcDNA3中的CMV启动子,构建了一种新的表达载体,使IL-6的表达水平提高了7.5倍。此外,我们还研究了pAdVAntage共转染对IL-6表达的影响,使之表达水平提高了3.6倍。以上结果为细胞因子在真核系统中的高效表达提供了有益的经验。

引用

页码：135 / 139

页数：5

共 8 条

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