提高人重组IL-6在真核细胞中表达的新途径

被引:3
作者
刘红涛
袁勇
张颖妹
王应
狄春辉
马大龙
机构
[1] 北京医科大学免疫系!北京
关键词
白细胞介素6; 基因转染; 内含子; COS-7细胞;
D O I
暂无
中图分类号
R392 [医学免疫学];
学科分类号
100102 ;
摘要
为提高IL-6在真核系统中的表达效率,我们在构建真核表达载体pcDNA3IL-6的基础上,用表达载体pCI中的CMV启动子和下游所带的β-球蛋白的第一个内含子,置换了pcDNA3中的CMV启动子,构建了一种新的表达载体,使IL-6的表达水平提高了7.5倍。此外,我们还研究了pAdVAntage共转染对IL-6表达的影响,使之表达水平提高了3.6倍。以上结果为细胞因子在真核系统中的高效表达提供了有益的经验。
引用
收藏
页码:135 / 139
页数:5
相关论文
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