基因组编辑:植物生物技术的机遇与挑战

被引:11
作者
程曦
王文义
邱金龙
机构
[1] 中国科学院微生物研究所植物基因组学国家重点实验室
关键词
序列特异性核酸酶; 基因组编辑; 植物基因工程;
D O I
10.13560/j.cnki.biotech.bull.1985.2015.03.016
中图分类号
Q943.2 [植物基因工程];
学科分类号
摘要
基于序列特异性核酸酶的基因组编辑技术可以在不同物种中对目标基因进行定点敲除,并可实现特定基因片段置换,基因的定点插入等基因组靶向修饰。基因组编辑是一种精准和高效的基因工程方法,近年来快速发展并得到了广泛的应用,并将改变生物技术的现状。目前,基因组编辑在不同植物,特别是农作物中的技术体系已建立,初步展示了其在植物生物技术领域的巨大潜力。介绍了不同基因组编辑系统的工作原理,并对基因组编辑技术在植物研究中的应用及成功案例进行了综述,最后对基因组编辑在植物生物技术领域所面临的机遇与挑战进行了讨论。
引用
收藏
页码:25 / 33
页数:9
相关论文
共 10 条
  • [1] Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System[J]. Zhen Liang,Kang Zhang,Kunling Chen,Caixia Gao.Journal of Genetics and Genomics. 2014(02)
  • [2] Creation of fragrant rice by targeted knockout of the OsBADH2 gene using TALEN technology
    Shan, Qiwei
    Zhang, Yi
    Chen, Kunling
    Zhang, Kang
    Gao, Caixia
    [J]. PLANT BIOTECHNOLOGY JOURNAL, 2015, 13 (06) : 791 - 800
  • [3] Improved soybean oil quality by targeted mutagenesis of the fatty acid desaturase 2 gene family
    Haun, William
    Coffman, Andrew
    Clasen, Benjamin M.
    Demorest, Zachary L.
    Lowy, Anita
    Ray, Erin
    Retterath, Adam
    Stoddard, Thomas
    Juillerat, Alexandre
    Cedrone, Frederic
    Mathis, Luc
    Voytas, Daniel F.
    Zhang, Feng
    [J]. PLANT BIOTECHNOLOGY JOURNAL, 2014, 12 (07) : 934 - 940
  • [4] Synthetic TAL effectors for targeted enhancement of transgene expression in plants
    Liu, Wusheng
    Rudis, Mary R.
    Peng, Yanhui
    Mazarei, Mitra
    Millwood, Reginald J.
    Yang, Jian-Ping
    Xu, Wenzhi
    Chesnut, Jonathan D.
    Stewart, Charles Neal, Jr.
    [J]. PLANT BIOTECHNOLOGY JOURNAL, 2014, 12 (04) : 436 - 446
  • [5] Rapid and Efficient Gene Modification in Rice and Brachypodium Using TALENs
    Shan, Qiwei
    Wang, Yanpeng
    Chen, Kunling
    Liang, Zhen
    Li, Jun
    Zhang, Yi
    Zhang, Kang
    Liu, Jinxing
    Voytas, Daniel F.
    Zheng, Xuelian
    Zhang, Yong
    Gao, Caixia
    [J]. MOLECULAR PLANT, 2013, 6 (04) : 1365 - 1368
  • [6] PhiC31 integrase facilitates genetic approaches combining multiple recombinases
    Monetti, Claudio
    Nishino, Koichiro
    Biechele, Steffen
    Zhang, Puzheng
    Baba, Takeshi
    Woltjen, Knut
    Nagy, Andras
    [J]. METHODS, 2011, 53 (04) : 380 - 385
  • [7] Targeted transgene integration in plant cells using designed zinc finger nucleases
    Cai, Charles Q.
    Doyon, Yannick
    Ainley, W. Michael
    Miller, Jeffrey C.
    DeKelver, Russell C.
    Moehle, Erica A.
    Rock, Jeremy M.
    Lee, Ya-Li
    Garrison, Robbi
    Schulenberg, Lisa
    Blue, Ryan
    Worden, Andrew
    Baker, Lisa
    Faraji, Farhoud
    Zhang, Lei
    Holmes, Michael C.
    Rebar, Edward J.
    Collingwood, Trevor N.
    Rubin-Wilson, Beth
    Gregory, Philip D.
    Urnov, Fyodor D.
    Petolino, Joseph F.
    [J]. PLANT MOLECULAR BIOLOGY, 2009, 69 (06) : 699 - 709
  • [8] The Repetitive DNA Elements Called CRISPRs and Their Associated Genes: Evidence of Horizontal Transfer Among Prokaryotes[J] . James S. Godde,Amanda Bickerton.Journal of Molecular Evolution . 2006 (6)
  • [9] High-frequency homologous recombination in plants mediated by zinc-finger nucleases
    Wright, DA
    Townsend, JA
    Winfrey, RJ
    Irwin, PA
    Rajagopal, J
    Lonosky, PM
    Hall, BD
    Jondle, MD
    Voytas, DF
    [J]. PLANT JOURNAL, 2005, 44 (04) : 693 - 705
  • [10] Site-specific recombinases: tools for genome engineering .2 Kilby N J,Snaith M R,Murray J A. Trends in genetics : TIG . 1993