硝基氟苯柱前衍生HPLC测定减肥药中芬氟拉明

A reversed-phase High Performance Liquid Chromatography method is developed for the determination of fenfluramine in slimming drug using precolumn derivatization.After eluted with chloroform,the samples were derivatized in solution of acetonitrile with water by 2,4-dinitrofluorobenzene at 40℃ for 60min in presence of Na2CO3 catalyst.The derivative obtained was determined on a Kromasil C18 column by using methanol-water （78:22,v/v） as mobile phase,and detection at 365 nm.The calibration curve is well linear in the range from 1.71～85.6 μg/mL with 0.9987 of correlation coefficient.The recoveries of the method range from 90.6 to 98.0% with a relative standard deviation less than 4% and the limit of detection 1.02 ng （S/N=3）.