漆酶高产菌株的筛选及产酶条件研究

We screened some high-yield fungi of laccase production by bavendamn's reaction and liquid ferment,and studied the conditions of laccase producing and activities of laccase in them.The results showed that 64 fungi had laccase activities and the Banvendamn's reaction were positive among the 71 fungi which we tested.The different fungi had different optimal carbon and nitrogen sources in their mediums.For example,Coriolus versicolor's optimal carbon and nitrogen sources were amylum and casein while Trametes trogii's were powder of wheatgrass and(NH 4) 2SO 4.The characters of different fungi were different.The optimum temperature and optimum pH of Coriolus versicolor's laccase reaction were 25℃ and 4.6 respectively,while Trame testrogii's were 30℃ and 4.0,respectively.K +and Zn 2+ increased activity whereas Ag +obviously inhibited it.