响应面法优化纳米二氧化硅固体分散技术辅助酶解制备染料木素

被引：16

作者：

金鑫 ^{[1
,2
]}

张振海 ^{[1
]}

朱静 ^{[1
,2
]}

孙娥 ^{[1
]}

郁丹红 ^{[1
,2
]}

陈小云 ^{[1
,2
]}

刘其媛 ^{[1
,2
]}

宁青 ^{[1
]}

贾晓斌 ^{[1
,2
]}

机构：

[1] 江苏省中医药研究院中药新型给药系统重点实验室

[2] 南京中医药大学

来源：

药学学报 | 2012年 / 04期

关键词：

染料木苷; 纳米二氧化硅; 固体分散体; 染料木素; 蜗牛酶;

D O I：

暂无

中图分类号：

TQ610 [一般性问题];

学科分类号：

070303 [有机化学];

摘要：

本研究旨在使用纳米二氧化硅固体分散技术,提高酶解制备染料木素的效率。首先采用溶剂法制备染料木苷-纳米二氧化硅固体分散体,利用差示扫描量热法和扫描电镜对固体分散体进行验证,再利用蜗牛酶将其水解从而制备染料木素。以染料木素转化率为指标,通过单因素试验分别考察染料木苷及其固体分散体在酶解过程中不同的影响因素,再利用响应面法优化染料木苷固体分散体酶解制备染料木素的工艺。染料木苷-纳米二氧化硅固体分散体酶解反应最佳条件:反应介质pH 7.1、温度为52.2℃、加酶量5.0 mg.mL—1、反应时间为7 h。在此条件下,转化率为(93.47±2.40)%,比未经形成固体分散体的染料木苷转化速率提高了2.62倍。对水解产物进行纯化得到单体染料木素。采用蜗牛酶水解染料木苷-纳米二氧化硅固体分散体制备染料木素,方法简单,酶解时间显著缩短,适用于规模化生产。

引用

页码：522 / 528

页数：7

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