超高效液相色谱串联质谱检测猪粪中残留的四环素类抗生素

被引：28

作者：

沈颖 ^{[1
]}

魏源送 ^{[1
]}

郭睿 ^{[1
]}

徐超一 ^{[2
]}

张朝晖 ^{[2
]}

周光漪 ^{[2
]}

赵鑫 ^{[2
]}

刘俊新 ^{[1
]}

机构：

[1] 中国科学院生态环境研究中心

[2] 北京出入境检验检疫局

来源：

环境化学 | 2009年 / 05期

关键词：

四环素类抗生素; 残留量; 检测; 猪粪; 超高效液相色谱;

D O I：

暂无

中图分类号：

S859.8 [兽医毒物学];

学科分类号：

0906 ;

摘要：

本研究建立了超高效液相色谱/四极杆-飞行时间串联质谱(UPLC/Q-ToFMS/MS)测定猪粪中残留土霉素、四环素和金霉素的方法.样品分别经McⅠlvaine-Na2 EDTA缓冲液和超声提取、SPE固相萃取后,采用超高效液相色谱分离,紫外检测外标法定量,四级杆-飞行时间串联质谱仪正离子模式定性.在0.5mg·kg-1DW—10mg·kg-1DW(dryweight)范围内,土霉素、四环素、金霉素的标准曲线R2均大于0.99,相对标准偏差小于3%,样品加标平均回收率在70.0%—113.0%之间;采用该方法,土霉素、四环素和金霉素的检测可在5min内完成,它们的检出限分别为0.1mg·kg-1,0.1mg·kg-1和0.2mg·kg-1.北京地区万头养猪场猪粪样品的检测结果表明,这3种四环素均有不同程度的检出,其中土霉素浓度范围10.5mg·kg-1DW—513.4mg·kg-1DW,四环素浓度范围12.53—77.10mg·kg-1DW,金霉素浓度范围0.0—19.22mg·kg-1DW.

引用

页码：747 / 752

页数：6

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