食品中沙门氏菌LAMP快速检测方法的建立

被引：38

作者：

黄金海 ^{[1
]}

孙跃辉 ^{[1
]}

陈瑞 ^{[2
]}

庄世文 ^{[1
]}

刘莹 ^{[1
]}

王静思 ^{[1
]}

机构：

[1] 天津大学化工学院

[2] 华南农业大学动物医学院

来源：

天津大学学报 | 2012年 / 45卷 / 05期

关键词：

沙门氏菌; 侵袭性因子A; 环介导等温扩增技术;

D O I：

暂无

中图分类号：

R155.5 [食品卫生与检验];

学科分类号：

摘要：

食品及其原料中沙门氏菌的快速、现场检测对食品安全控制具有重要意义.根据沙门氏菌invA基因核苷酸序列设计一组引物,应用环介导等温扩增技术(LAMP),分别对7种不同血清型沙门氏菌和4种非沙门氏菌进行扩增,同时建立沙门氏菌人工污染的食品模型,比较了LAMP法与活菌计数检测的敏感性.结果表明,该方法仅对沙门氏菌产生特异性扩增,灵敏度高达336,mL-1,食品样品经细菌富集培养后,检测灵敏度高达8.25,g-1.所建立的检测沙门氏菌方法具有较高的特异性与灵敏性,操作简单、快速,可用于沙门氏菌污染食品的快速检测.

引用

页码：468 / 472

页数：5

共 10 条

[1] 食品中沙门氏菌PCR快速检测方法的建立
钟伟军
赵明秋
张彩虹
陈金顶
[J]. 中国人兽共患病学报, 2007, (12) : 1216 - 1221
[2] 沙门氏菌的invA基因序列分析与分子检测
陈金顶
索青利
廖明
辛朝安
[J]. 中国人兽共患病杂志, 2004, (10) : 868 - 871
[3] 畜产品中沙门氏菌污染的检测与控制
曾晓芳
[J]. 四川畜牧兽医, 2003, (04) : 28 - 29
[4] Development and validation of a rapid real-time PCR based method for the specific detection of Salmonella on fresh meat
McGuinness, Sheila
McCabe, Evonne
O'Regan, Edel
Dolan, Anthony
Duffy, Geraldine
Burgess, Catherine
Fanning, Seamus
Barry, Thomas
O'Grady, Justin
[J]. MEAT SCIENCE, 2009, 83 (03) : 555 - 562
[5] Loop-mediated isothermal amplification for the rapid, sensitive, and specific detection of the O9 group of Salmonella in chickens[J] . Masashi Okamura,Yousuke Ohba,Shuichi Kikuchi,Akiko Suzuki,Hajime Tachizaki,Kazuaki Takehara,Masanari Ikedo,Tadashi Kojima,Masayuki Nakamura.Veterinary Microbiology . 2008 (1)
[6] Loop-mediated isothermal amplification for discriminating between human herpesvirus 6 A and B[J] . Masaru Ihira,Akane Ohta,Ken Sugata,Sadao Suga,Yoshizo Asano,Tetsushi Yoshikawa.Journal of Virological Methods . 2008 (1)
[7] Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification （RT-LAMP）[J] . Kelly A. Curtis,Donna L. Rudolph,S. Michele Owen.Journal of Virological Methods . 2008 (2)
[8] Salmonella contamination in pigs at slaughter and on the farm: A field study using an antibody ELISA test and a PCR technique[J] . International Journal of Food Microbiology . 2007 (3)
[9] Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation
Mori, Y
Nagamine, K
Tomita, N
Notomi, T
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 289 (01) : 150 - 154
[10] The use of a PCR-generated inv A probe for the detection of Salmonella spp. in artificially and naturally contaminated foods[J] . Michael Bülte,Peter Jakob.International Journal of Food Microbiology . 1995 (3)

← 1 →