IL-12对CIK细胞体外增殖及细胞毒活性影响的研究

被引：5

作者：

秦莉

王志华

袁玉涛

机构：

[1] 哈尔滨医科大学附属肿瘤防治研究所

来源：

国际免疫学杂志 | 2007年 / 04期

关键词：

细胞因子诱导的杀伤细胞; 白介素-12; 增殖能力; 细胞毒性;

D O I：

暂无

中图分类号：

R [医药、卫生];

学科分类号：

10 ;

摘要：

目的观察IL-12对细胞因子诱导的杀伤细胞(CIK)体外扩增和细胞毒活性的影响。方法采用3种不同的细胞因子组合扩增CIK细胞,即IL-2组:IFN-γ、CD3单抗、IL-1和IL-2;IL-2加IL-12组:IFN-γ、CD3单抗、IL-1、IL-2和IL-12;IL-12组:IFN-γ、CD3单抗、IL-1和IL-12。细胞计数法测定细胞的增殖、流式细胞术分析细胞表型,MTT法测定细胞毒作用。结果3种方法均可使细胞增殖能力显著增加,对CD3+CD56+细胞的诱导作用也无明显差异,IL-12与IL-2联合作用组促增殖能力和细胞毒性作用明显强于其他两组,P<0.05,差异有统计学意义。结论IL-12同样可以用于CIK细胞的诱导,而联合IL-12与IL-2可以显著增强CIK细胞的增殖能力和细胞毒性。

引用

页码：203 / 207

共 8 条

[1] 细胞因子诱导的杀伤细胞的研究进展 [J].

袁玉涛 .

国际免疫学杂志, 2007, (03) :163-166

[2] The apoptotic and proliferative fate of cytokine-induced killer cells after redirection to tumor cells with bispecific Ab [J].

Kornacker, M ;

Verneris, MR ;

Kornacker, B ;

Ganten, TM ;

Scheffold, C ;

Negrin, RS .

CYTOTHERAPY, 2006, 8 (01) :13-23

[3]

Cytokine-induced Killer Cells: NK-like T Cells with Cytotolytic Specificity against Leukemia[J] . Y. C. Linn,Kam M. Hui.Leukemia & Lymphoma . 2003 (9)

[4]

Production of interferon-gamma by tumor-sensitized T cells is essential for interleukin-12–induced complete tumor eradication[J] . Natalie C Lee,Kangla Tsung,Jeffrey A Norton.Surgery . 2002 (2)

[5]

The Direct Effect of IL-12 on Tumor Cells: IL-12 Acts Directly on Tumor Cells to Activate NF-κB and Enhance IFN-γ-Mediated STAT1 Phosphorylation[J] . Wei Su,Toshinori Ito,Tsukasa Oyama,Toru Kitagawa,Takao Yamori,Hiromi Fujiwara,Hikaru Matsuda.Biochemical and Biophysical Research Communications . 2001 (2)

[6] Tumor cell responses to IFNγ affect tumorigenicity and response to IL-12 therapy and antiangiogenesis [J].

Coughlin, CM ;

Salhany, KE ;

Gee, MS ;

LaTemple, DC ;

Kotenko, S ;

Ma, XJ ;

Gri, G ;

Wysocka, M ;

Kim, JE ;

Liu, L ;

Liao, F ;

Farber, JM ;

Pestka, S ;

Trinchieri, G ;

Lee, WMF .

IMMUNITY, 1998, 9 (01) :25-34

[7]

THE INTERLEUKIN-12/INTERLEUKIN-12-RECEPTOR SYSTEM: Role in Normal and Pathologic Immune Responses[J] . Maurice K. Gately,Louis M. Renzetti,Jeanne Magram,Alvin S. Stern,Luciano Adorini,Ueli Gubler,David H. Presky.Annual Review of Immunology . 1998 (1)

[8]

Enhanced antitumor effect of oncolytic adenovirus expressing interleukin-12 and B7-1 in an immunocompetent murine model .2 Lee YS,Kim JH,Choi KJ,Choi IK,Kim H,Cho S et al. Clin Cancer Res . 2006

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