大鼠尾椎间盘针刺退变模型的建立

被引：9

作者：

韩新立 ^{[1
]}

宋洁富 ^{[2
]}

机构：

[1] 山西医科大学第一临床医学院骨科

[2] 山西省人民医院骨科

来源：

医学研究杂志 | 2009年 / 38卷 / 03期

关键词：

椎间盘; 退变; 动物模型; 大鼠;

D O I：

暂无

中图分类号：

R-332 [医用实验动物学]; R245 [针灸学、针灸疗法];

学科分类号：

100402 [劳动卫生与环境卫生学]; 100517 [针灸学];

摘要：

目的建立大鼠尾椎间盘针刺退变模型并进行X线和组织学观察。方法Wistar大鼠36只,使用20G皮肤穿刺针对Co7,8Co8,9椎间盘分别随机行腹背横贯或半横贯穿刺一针,Co9,10椎间盘不穿刺作为对照,术前及术后1、2、4周时对造模后的椎间盘及对照的椎间盘(Co9,10)行X线检查及HE染色观察。结果术后第1周到第4周,穿刺后的椎间盘高度指数呈现持续减小;组织学检查发现横贯穿刺和半横贯穿刺椎间盘1周时都发生退变,而横贯穿刺退变较严重(P<0.01)。结论横贯或半横贯针刺方法可以成功建立大鼠尾椎间盘退变模型,方法可靠、有效、重复性好。

引用

收藏

页码：83 / 86

页数：4

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