氟美松对嗜酸粒细胞凋亡的作用及其机制

被引：15

作者：

赖克方

王长征

郭先健

钱桂生

机构：

[1] 广州呼吸疾病研究所!,第三军医大学新桥医院全军呼吸研究所,第三军医大学新桥医院全军呼吸研究所,第三军医大学新桥医院全军呼吸研究所

来源：

中华内科杂志 | 2000年 / 02期

关键词：

地塞米松; 嗜酸粒细胞; 脱噬作用; 白细胞介素-5; 基因,bcl-2;

D O I：

暂无

中图分类号：

R562.25 [];

学科分类号：

1002 ; 100201 ;

摘要：

目的　探讨氟美松对体外嗜酸粒细胞凋亡的作用及其机制。方法　在不同浓度白细胞介素 (IL) 5和氟美松的条件下体外培养嗜酸粒细胞 ,锥虫蓝拒染法和原位凋亡检测法观察嗜酸粒细胞凋亡的变化及嗜酸粒细胞凋亡抑制基因bcl 2表达的变化。结果　IL 5显著抑制嗜酸粒细胞凋亡 ,氟美松呈浓度和时间依赖性抑制IL 5介导的嗜酸粒细胞存活 ,10 -6mol/L氟美松显著抑制 1× 10 4 U/LIL 5介导的嗜酸粒细胞存活 ,培养 3d嗜酸粒细胞存活率为 (7± 3) % (P <0 .0 1) ,抑制率达 (90± 4) %。IL 5使嗜酸粒细胞bcl 2表达增强 ,氟美松对此具有抑制作用。但高浓度的IL 5 (1× 10 6U/L)可抑制氟美松的凋亡诱导作用。结论　氟美松具有诱导嗜酸粒细胞凋亡的作用 ,其机制可能与抑制嗜酸粒细胞bcl 2表达有关。

引用

页码：38 / 41+72

页数：5

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