结核分枝杆菌耐链霉素和乙胺丁醇分子机制的研究

被引:2
作者
张向晖
李奇凤
张辉
袁俐
机构
[1] 石河子大学医学院
关键词
结核分枝杆菌; 链霉素; 乙胺丁醇; 耐药性;
D O I
10.13241/j.cnki.pmb.2008.12.058
中图分类号
R52 [结核病];
学科分类号
摘要
目的:研究结核分枝杆菌耐链霉素和乙胺丁醇的rpsL和emb B基因突变情况,探讨耐药基因突变与耐药性的关系。方法:通过传统药敏实验和聚合酶链反应(PCR)--单链构象多态性(SSCP)技术初步鉴定62株临床分离株的药敏和rps L、emb B基因。结果:与结核菌标准株H37Rv对照,分析30例TB菌耐链霉素(SM)的rps L基因,发现其突变率为70.0%(21/30),分析29例耐乙胺丁醇(EMB)的emb B基因,该基因的突变率为65.5%(19/29)。结论:部分结核分枝杆菌耐SM和EMB是由于其rps L、emb B基因突变所致,PCR-SSCP银染技术可能成为测定部分结核分枝杆菌耐药的简便、快速的方法。
引用
收藏
页码:2272 / 2274
页数:3
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