BIOLOGICAL FEATURES OF HUMAN T-ACTIVATED KILLER CELLS

被引:22
作者
魏虎来
苏海翔
姚小健
机构
关键词
T-activated killer (T-AK) cell; Cytomorphology; Phenotype; Cytotoxicity; Heterogeneity;
D O I
暂无
中图分类号
R730.2 [肿瘤病理学、病因学];
学科分类号
100214 ;
摘要
Objective: To investigate the immunobiological essence of T’activated killer (T-AK) cells induced byanti-CD, monoclonal antibody (CD3McAb) andrecombinant interleukin-2 (rIL-2) co-stimulation.Methods: The cytomorphology, t phenotype andcytotoxicity of T-AK cells generated from humanperipheral blood mononuclear cells (PBMC) weredetermined. Results: T-AK cells were similar toactivated lymphoblasts in morphology, more than 90%of T-AK cells expressed the phenotypes 6f Tlymphocytes (CD,+, CD,+), and 20%--50% of the cellswere NK-like phenotype (CD,.+, CD,.+), some of themexpressed IL-2 receptor (CDZ,+), CD38 antigen (CD,,+)and MHC-II antigen (HLA-DR+) characteristic marksfor the activated T lymphocytes. T-AK cells attackingtargets were morphologically large volumes withgranules and mainly contained CD,+ and CD,.+ cells. TAK cells possessed high tumoricidal activities againstNK-sensitive K562 cells and NK-resistant Ran cells, thecytotoxicity was composed of mainly CD,McAbactivated CD,AK activity (~50%), IL-2 induced LAKactivity (-30%), NK activity (~10%) and the activities ofinhibitory factors in T-AK supernatant (~10%).Conclusion: T-AK cells are a heterogeneous cell.population consisting of mainly activated T lymphocytesand NK-like cells, the main part of T-AK cytotoxicity isthe common activities of CD,AK cells and LAK cells.
引用
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页码:111 / 114
页数:4
相关论文
共 3 条
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