现代非培养技术在反硝化微生物种群结构和功能研究中的应用

被引：5

作者：

梁丽华

左剑恶

机构：

[1] 清华大学环境科学与工程系

来源：

环境科学学报 | 2008年 / 04期

关键词：

反硝化微生物; 功能基因; 种群结构; 非培养技术;

D O I：

10.13671/j.hjkxxb.2008.04.016

中图分类号：

X172 [环境微生物学]; X703 [废水的处理与利用];

学科分类号：

071012 ; 0713 ; 083002 ;

摘要：

反硝化过程对于废水生物脱氮工艺的运行、土壤肥分的流失以及N2O的排放均具有重要意义,但参与反硝化过程的微生物种类繁多且多数不可培养,导致对自然环境中反硝化微生物的种群结构及功能的研究具有很大难度.现代非培养分子技术的发展使得对反硝化微生物进行原位、准确、全面的研究成为可能.对反硝化功能基因进行指纹图谱分析、定量PCR或者利用FISH等技术可以有效确定反硝化菌的组成和数量,通过检测反硝化酶和mRNA可将反硝化菌的种群鉴定与代谢活性联系起来,最近新出现的同位素底物标记技术甚至可直接确定反硝化菌的碳源利用情况.重点介绍了上述各种现代非培养技术对反硝化细菌种群结构和功能的研究现状,以期为深入了解反硝化微生物的多样性和功能特性提供参考.

引用

收藏

页码：599 / 605

页数：7

相关论文

共 14 条

[1] 反硝化真菌-细菌优化组合及其脱氮能力研究 [J].

李平 ;

张山 ;

郑永良 ;

江月 ;

陈舒丽 ;

邹煜平 ;

刘德立 .

环境科学与技术, 2006, (03) :9-10+45+115

[2]

新型生物脱氮理论与技术[M]. 科学出版社 , 郑平等编著, 2004

[3] Identification of acetate- or methanol-assimilating bacteria under nitrate-reducing conditions by stable-isotope probing [J].

Osaka, Toshifumi ;

Yoshie, Sachiko ;

Tsuneda, Satoshi ;

Hirata, Akira ;

Iwami, Norio ;

Inamori, Yuhei .

MICROBIAL ECOLOGY, 2006, 52 (02) :253-266

[4] TGGE analysis of the diversity of ammonia-oxidizing and denitrifying bacteria in submerged filter biofilms for the treatment of urban wastewater [J].

Gomez-Villalba, B. ;

Calvo, C. ;

Vilchez, R. ;

Gonzalez-Lopez, J. ;

Rodelas, B. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 72 (02) :393-400

[5] Changes in bacterial community structure correlate with initial operating conditions of a field-scale denitrifying fluidized bed reactor [J].

Hwang, C. ;

Wu, W. -M. ;

Gentry, T. J. ;

Carley, J. ;

Carroll, S. L. ;

Schadt, C. ;

Watson, D. ;

Jardine, P. M. ;

Zhou, J. ;

Hickey, R. F. ;

Criddle, C. S. ;

Fields, M. W. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 71 (05) :748-760

[6] Finding the missing link between diversity and activity using denitrifying bacteria as a model functional community [J].

Philippot, L ;

Hallin, S .

CURRENT OPINION IN MICROBIOLOGY, 2005, 8 (03) :234-239

[7] Denitrifiers associated with methanotrophs and their potential impact on the nitrogen cycle [J].

Knowles, R .

ECOLOGICAL ENGINEERING, 2005, 24 (05) :441-446

[8] Design of 16S rRNA-targeted oligonucleotide probes and microbial community analysis in the denitrification process of a saline industrial wastewater treatment system [J].

Yoshie, S ;

Noda, N ;

Tsuneda, S ;

Hirata, A ;

Inamori, Y .

FEMS MICROBIOLOGY LETTERS, 2004, 235 (01) :183-189

[9] Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR [J].

Henry, S ;

Baudoin, E ;

López-Gutiérrez, JC ;

Martin-Laurent, F ;

Brauman, A ;

Philippot, L .

JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (03) :327-335

[10] Detection and quantification of copper-denitrifying bacteria by quantitative competitive PCR [J].

Qiu, XY ;

Hurt, RA ;

Wu, LY ;

Chen, CH ;

Tiedje, JM ;

Zhou, Z .

JOURNAL OF MICROBIOLOGICAL METHODS, 2004, 59 (02) :199-210