蛋白质组学研究中的双向电泳技术

被引:29
作者
廖翔
应天翼
黄留玉
黄培堂
机构
[1] 军事医学科学院生物工程研究所
[2] 军事医学科学院生物工程研究所 北京
[3] 北京
关键词
蛋白质组学; 双向电泳;
D O I
暂无
中图分类号
Q51 [蛋白质];
学科分类号
071010 ; 081704 ;
摘要
蛋白质组学研究已经成为后基因组时代的研究热点,其两大支柱是双向凝胶电泳技术和生物质谱技术。尽管双向电泳技术近几年已经取得了突破性进展,是当前蛋白质分离的最常用技术,但其本身还有一些难以克服的问题。随着质谱技术的快速发展,双向电泳逐渐成为蛋白质组学研究的瓶颈。本文综述双向电泳主要技术步骤的现状、存在问题及其改进方向。
引用
收藏
页码:522 / 524
页数:3
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