大豆主栽品种体细胞胚胎发生的影响因素及再生植株

被引：9

作者：

邓向阳

卫志明

许智宏

机构：

[1] 中国科学院上海植物生理研究所,中国科学院上海植物生理研究所,中国科学院上海植物生理研究所上海,上海,上海

来源：

实验生物学报 | 2000年 / 01期

关键词：

大豆; 体细胞胚发生; 植株再生;

D O I：

暂无

中图分类号：

S565.1 [大豆];

学科分类号：

0901 ;

摘要：

以大豆3个主栽品种3.0—6.0mm幼胚的子叶为外植体研究体外体胚发生的各影响因素。发现不仅激素种类、浓度及其组合而且幼胚长度、接种量等因素对体胚发生方式有重要影响。通过因素方差分析实验表明除幼胚长度、激素浓度、基本培养基种类对胚性反应频率有极其显著作用外,且发现前两种因素存在交互作用,并刚好达到极其显著水平（1%）,最佳2.4-D浓度与幼胚长度组合为10D/4.0mm;20D/5.0mm;40D/5.0mm。在此组合下,基本培养基E1明显优于MS。总之,在我们建立的再生系统中,大豆未成熟子叶的体胚发生频率已达50%以上,由体胚正常萌发成植株的频率达52.9%—62.6%。

引用

页码：69 / 76+78 +78-79

页数：10

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