酶抗体应用实现热启动PCR

被引：3

作者：

林晴 ^{[1
]}

许木于 ^{[2
]}

李庆阁 ^{[2
]}

机构：

[1] 福建中医学院

[2] 厦门大学生命科学学院

来源：

中国生物化学与分子生物学报 | 2008年 / 05期

关键词：

热启动; 抗体; PCR;

D O I：

10.13865/j.cnki.cjbmb.2008.05.011

中图分类号：

Q503 [生物化学技术];

学科分类号：

071010 ; 081704 ;

摘要：

通过制备酶抗体,形成抗原抗体复合物,使得酶在较高的温度95℃,1min,得以释放发挥聚合活性,实现热启动PCR.建立的热启动PCR体系灵敏度高,可以检测到1个拷贝的DNA分子,较普通PCR体系提高了3个数量级,并且具有较好的线性关系.这种通过形成抗原抗体复合物达到热启动PCR目的的方法的建立,具有着重要的意义,是提高PCR灵敏度和特异性的重要方法之一.

引用

页码：475 / 480

页数：6

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