丝组二肽所含基团在其切割DNA反应中的作用

被引：5

作者：

万荣

王宁

赵刚

赵玉芬

机构：

[1] 清华大学生命科学与工程研究院教育部生命有机膦化学开放实验室!北京

[2] 清华大学生命科学与工程研究院教育部生命有机膦

来源：

高等学校化学学报 | 2000年 / 12期

关键词：

丝组二肽; DNA的切割; 氨基酸侧链效应; 切割机理;

D O I：

暂无

中图分类号：

O629 [天然化合物];

学科分类号：

070303 ; 081704 ;

摘要：

The effects of the amino, hydroxyl, imidazole and carboxyl groups of Ser His on DNA cleavage reaction were studied. It was found that the amino and hydroxyl groups were essential for the DNA cleavage activity of Ser His, and that anyone of them blocked would result into the loss of cleavage activity. The carboxyl group was not the necessary group. The presence of the imidazole group would enhance the cleavage activity. However, the histidyl serine dipeptide, as the formula isomer of Ser His with the inverse peptide sequence, could not cleave DNA at all. Based on the above experimental results, a presumed cleavage mechanism of Ser His on DNA was proposed. [WT5HZ]

引用

页码：1864 / 1866

页数：3