脉冲场凝胶电泳技术在鼠伤寒沙门菌分型中的应用

被引:7
作者
纪惠玲
陈艳
刘秀锋
李伟
阚飙
机构
[1] 福州市疾病预防控制中心
[2] 中国疾病预防控制中心传染病预防控制所
关键词
鼠伤寒沙门菌; 脉冲场电泳; 分子分型;
D O I
暂无
中图分类号
R378.2 [肠道杆菌];
学科分类号
摘要
[目的]探讨脉冲场凝胶电泳(PFGE)在鼠伤寒沙门菌分型中的应用。[方法]选择XbaⅠ酶对鼠伤寒沙门菌全基因组DNA进行酶切、脉冲场凝胶电泳,电泳带用Freeview软件进行相似性比较。[结果]13株鼠伤寒沙门菌经PF GE,根据其条带的差异可分为10个PFGE型别。[结论]PFGE法重复实验结果稳定,分型力较强,适合鼠伤寒沙门菌分型应用。
引用
收藏
页码:3 / 5
页数:3
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