用变性梯度凝胶电泳分析PCR克隆的突变率

Random mutagenesis fo gene-sized DNA molecules by use of PCR with Taq DNA polymerase. Zhou Yuhong,Zhang Xiaoping and Ebright RH. Nucleic Acids Research . 1991

DNA fragments differing by single base-pair substitutions separated in denaturing gradient gels: correspondence with melting theory. Fishcer SG,Lerman LS. Proceedings of the National Academy of Sciences of the United States of America . 1983

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Drumm MD,Saulino M. Nucleic Acids Research . 1991

A denaturing gradient gel electrophoresis assay for sensitive detection of P53 mutation. Beck S,Kwitek AE,Cogen PH et al. Human Genetics . 1993

Clark JM. Nucleic Acids Research . 1988

Random mutagenesis fo gene-sized DNA molecules by use of PCR with Taq DNA polymerase. Zhou Yuhong,Zhang Xiaoping and Ebright RH. Nucleic Acids Research . 1991

DNA fragments differing by single base-pair substitutions separated in denaturing gradient gels: correspondence with melting theory. Fishcer SG,Lerman LS. Proceedings of the National Academy of Sciences of the United States of America . 1983

Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Drumm MD,Saulino M. Nucleic Acids Research . 1991

A denaturing gradient gel electrophoresis assay for sensitive detection of P53 mutation. Beck S,Kwitek AE,Cogen PH et al. Human Genetics . 1993

Clark JM. Nucleic Acids Research . 1988