Crystal structure of Streptomyces diastaticus No. 7 strain M1033 xylose isomerase

被引:1
作者
朱学勇
龚为民
牛立文
滕脉坤
徐庆平
伍传金
崔涛
王玉珍
王淳
机构
[1] Young Scientist Laboratory of Structure Biology
[2] Department of Biology
[3] University of Science and Technology of China
[4] Hefei
[5] China
[6] State Key Laboratory of Bionmacromolecules
[7] Institute of Biophysics
[8] Chinese Academy of Sciences
[9] Beijing
[10] China
[11] Department of Biology
[12] University of Science and Technology of China
[13] Hefei
关键词
xylose isomerase; glucose isomerase; streptomyces; crystal structure;
D O I
暂无
中图分类号
Q41 [普通生理学];
学科分类号
071003 ;
摘要
<正> The crystal structures of Streptomyces diastaticus No. 7 strain M1033 xylose isomerase (SDXyI) have been analysed and refined at 0.19nm. The crystal space group is I222, with unit cell dimensions of a=9.884 ran, b=9.393nm and c=8.798nm. Based on the coordinates of the Streptomyces rubiginosus xylose isomerase (SRXyI), the initial model of SDXyl was built up by the dose packing analysing and R-factor searching and refined by PROLSQ to a final R-factor of 0.177 with the rms deviations of bond lengths and bond angles of 0.001 9nm and 2.1°, respectively. No significant global conformation change existed between SRXyI and SDXyI except the local conformation in the active site.
引用
收藏
页码:636 / 644
页数:9
相关论文
共 2 条
[1]  
Transformations of optimize the superposition of similar structures. Hendrickson,W. A. Acta Crystallographica . 1979
[2]  
Traitement statistique des erreurs dans la determination des structures cristallines. Luzzati,V. Acta Crystallographica . 1952