枸杞胚乳愈伤组织细胞的悬浮培养及无丝分裂的活体观察

被引：7

作者：

顾淑荣

桂耀林

刘淑琼

徐廷玉

机构：

[1] 中国科学院植物研究所,中国科学院植物研究所,中国科学院植物研究所,中国科学院植物研究所北京,北京,北京,北京

来源：

Journal of Integrative Plant Biology | 1991年 / 06期

关键词：

枸杞; 胚乳愈伤组织; 细胞悬浮培养; 劈裂式无丝分裂; 植株再生;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

<正> The endosperm calli were induced on MS basic medium supplemented with lppm 2,4-D,0.5ppm KT and 5% sucrose.The medium which contained lppm BAP,0.1ppm NAA and 2%sucrose was used for cell suspension culture.In suspension cell culture,amitosis of cleavagedivision of nucleus have been observed after 5 days of culture.First the nuclear membraneand nucleolus disappeared.The crevice appeared in the center of the nucleus,and the nucleusdivided into two daughter nuclei of similar size and each with a nucleolus.The daughter nu-cleus resembled an eye in shape.FoIlowing;the emergence of cell wall,the two new unequalcells were produced.Such amitotic division proceeded repeatedly until the callus developedand eventually plantlet regenerated,

引用

页码：478 / 481+499 +499

页数：5