氯化甲基汞对发育阶段大鼠小脑转录因子CREB DNA结合活性的影响

被引：3

作者：

郭杰

董丽波

张春霞

李志超

机构：

[1] 吉林大学公共卫生学院毒理学教研室

[2] 中国预防医学科学院环境卫生监测所

来源：

吉林大学学报(医学版) | 2002年 / 04期

关键词：

氯化甲基汞; CREBDNA结合活性; 发育脑;

D O I：

10.13481/j.1671-587x.2002.04.004

中图分类号：

R994 [毒物学分支];

学科分类号：

摘要：

目的 :探讨氯化甲基汞 ( MMC)对发育大鼠小脑组织转录因子 CREB DNA结合活性的影响。方法 :将妊娠大鼠于妊娠 7～ 1 0 d连续 4d每日灌胃给予氯化甲基汞 4mg· kg-1,取生后 1、3、7、1 4d大鼠小脑组织提取核蛋白。采用凝胶移位法观察 MMC对发育阶段大鼠小脑转录因子CREB DNA结合活性的影响。结果 :对照组和实验组各发育阶段小脑组织 CREB在凝胶移位电泳中均呈现两条迟滞带 ;对照组和实验组 P1 - 7大鼠幼仔小脑 CREB DNA结合活性随生后发育时间延长呈下降趋势 ;实验组大鼠幼仔小脑 CREB DNA结合活性均高于相应对照组。结论 :CREB参与大鼠脑发育的调节 ;甲基汞所致发育脑组织损伤可能与其引起 CREB DNA结合活性升高有关。

引用

页码：338 / 340

页数：3

共 11 条

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