猪繁殖与呼吸综合征病毒cDNA文库的构建及基因组序列的测定

被引：3

作者：

唐勇

陈焕春

黄勤锋

姚清侠

赵武

机构：

[1] 华中农业大学动物医学院动物病毒室,华中农业大学动物医学院动物病毒室,华中农业大学动物医学院动物病毒室,华中农业大学动物医学院动物病毒室,华中农业大学动物医学院动物病毒室湖北武汉,暨南大学生命科技学院生物工程系,广东广州,,湖北武汉,,湖北武汉,,湖北武汉,,湖北武汉,

来源：

中国病毒学 | 2005年 / 02期

关键词：

猪繁殖与呼吸综合征病毒; cDNA文库; 基因组; 测序;

D O I：

暂无

中图分类号：

S852.65 [家畜病毒学];

学科分类号：

090601 ;

摘要：

In this study, the RNA of PRRSV HN1 strain was isolated and the cDNA fragments were amplified by RT-PCR. These fragments were then inserted to T-vector and constructed the cDNA library. The genome of PRRSV HN1 strain was sequenced and submitted to the GenBank obtained the ID: AY457635. The homology between this HN1 and other strains was analyzed. HN1 strain has the lowest homology with the Netherlands isolate LV which is 56.0%, and has the highest homology with the USA isolate MLV and VR2332 which is 99.1% and 99.0% respectively. The HN1 strain and MLV and VR2332 are on the same evolution branch, it proved that the HN1 strain is North America strain. In China, the HN1 strain has the highest homology with Beijing isolate BJ-4 (99.0%) and the lowest homology with another Beijing isolate HB2 (89.0%).

引用

页码：203 / 205

页数：3