肿瘤坏死因子-α在大鼠脑缺血再灌注损伤中的作用机制

被引：19

作者：

张莉莉

王景周

周华东

陈曼娥

机构：

[1] 第三军医大学附属大坪医院脑血管一科

来源：

中华老年医学杂志 | 2003年 / 02期

关键词：

肿瘤坏死因子; 脑缺血发作,暂时性; 再灌注损;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

摘要：

目的　探讨肿瘤坏死因 α(TNF α)在大鼠脑缺血再灌注损伤中的作用机制 ,尝试应用抗TNF α单抗进行缺血后的脑保护治疗。　方法　分别应用免疫组化方法和分光光度计比色法监测经大脑中动脉闭塞 2h后tor大鼠脑缺血再灌注不同时点TNF α的表达规律及髓过氧化物酶 (MPO)活性 ,并应用四氮唑红 (TTC)染色法测量再灌注 2 4h脑梗死灶体积。　结果　脑缺血再灌注后 2 4hTNF α表达和白细胞活性均达到高峰〔分别为 (3 7 86± 9 78)个和 (0 2 90± 0 0 71)U/ g湿组织 ;对照组分别为 (1 83± 1 41)个和 (0 0 16± 0 0 0 3 )U/ g湿组织〕 ,二者呈现同步变化。应用抗TNF α抗体可减少白细胞聚集〔治疗组为 (0 148± 0 0 3 3 )U/g湿组织〕和脑梗死灶体积〔手术组为 (15 8 7± 8 1)mm3,治疗组为 (86 3± 12 2 )mm3〕。　结论　TNF α通过炎性反应参与了缺血再灌注脑损伤 ,应用抗TNF α抗体可起到缺血性脑保护作用

引用

页码：37 / 39

页数：3

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