雌二醇的水凝胶毛细管电泳免疫分析

被引：7

作者：

苏萍

王永成

张新祥

孙玲

常文保

机构：

[1] 北京大学化学与分子工程学院!北京

来源：

高等学校化学学报 | 2000年 / 06期

关键词：

水凝胶; 毛细管电泳; 激光诱导荧光; 免疫分析; 雌二醇;

D O I：

暂无

中图分类号：

O657.8 [毛细管分析、电毛细管分析];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, a kind of thermally linear polymer, poly N isopropylacrylamide(PNIPA) was packed into capillary electrophoresis, which can be used to separate bound and free estradiol antigen. A useful method for determination of estradiol in serum by hydrogel capillary electrophoretic immunoassay(CEIA) was developed. The influence of concentration and pH of buffer, concentration of hydrogel, applied voltage, temperature etc has been studied. The limit of detection and the linear range of estrodiol were 30 pg/mL and 0 1—10 ng/mL. [WT5HZ]

引用

页码：870 / 872

页数：3