原花青素对人结肠癌细胞SW620增殖和凋亡的影响

被引：18

作者：

何佳声 ^{[1
]}

黄学锋 ^{[2
]}

机构：

[1] 浙江省富阳市第二人民医院外科

[2] 浙江大学医学院附属邵逸夫医院肛肠科

来源：

中国中西医结合外科杂志 | 2010年 / 16卷 / 04期

关键词：

结肠癌; 葡萄籽提取物原花青素; 增殖; 凋亡;

D O I：

暂无

中图分类号：

R285.5 [中药实验药理]; R735.35 [];

学科分类号：

1008 ; 100214 ;

摘要：

目的:观察葡萄籽提取物原花青素对人结肠癌细胞株SW620细胞增殖和凋亡的影响。方法:SW620细胞与20、40、60、80μg/mL的原花青素共同孵育24h～8d,采用噻唑蓝(MTT)比色法检测细胞增殖抑制率,流式细胞术分析细胞凋亡情况,分光光度法检测Caspase-3酶活性。结果:不同浓度(20～80μg/mL)的原花青素对SW620细胞的生长有明显的抑制作用,呈现出一定的剂量依赖性;含原花青素20、40、80μg/mL的观察组细胞凋亡发生率分别为6.9%、18.6%及22.9%,不含药的对照组细胞凋亡发生率为1.3%。SW620细胞与含药(40μg/mL)或不含药的培养基共同培养48h或72h,对照组细胞几乎无法检出Caspase-3酶活性,而给药组细胞活性显著增加。结论:原花青素在体外可呈浓度依赖性抑制人结肠癌细胞株SW620的增殖并通过Caspase-3通路促进其凋亡。

引用

页码：439 / 442

页数：4

共 7 条

[1]

Anti-angiogenic efficacy of grape seed extract in endothelial cells. Agarwal C,Singh RP,Dhanalakshmi S,et al. Oncology Reports . 2004

[2]

Dietary feeding of proanthocyanidins from grape seeds prevents photocarcinogenesis in SKH-1 hairless mice: relationship to decreased fat and lipid peroxidation. Mittal A,Elmets CA,Katiyar SK. Carcinogenesis . 2003

[3]

Grape seed proanthocyanidins promote apoptosis in human epidermoid carcinoma A431 cells through alterations in Cdki-Cdk-cyclin cascade, and caspase-3 activation via loss of mitochondrial membrane potential. Meeran SM,Katiyar SK. Experimental Dermatology . 2007

[4]

Proanthocyanidins and tannin-like compounds-nature, occurrence, dietary intake and effects on nutrition and health. Celestino Santos-Buelga,Augustin Scalbert. Journal of the Science of Food and Agriculture . 2000

[5]

Procyanidin oligomers from Japanese quince(Chaenomeles japonica)fruit inhibit activity of MMP-2and MMP-9metalloproteinases. STREK M,GORLACH S,PODSEDEK A. Agricultural Food Chemistry . 2007

[6]

Ditary grape-seed proanthocyanidin inhibition of ultraviolet B-induced immune suppression is associated with induction of IL-12. SHARMA S D,KATIYAR S K. Carcinogenesis . 2006

[7]

Proanthocyanidins inhibit mitogenic and survival-signalling in vitro and tumor growth in vivo. MEERAN S M. Frontiers in Bioscience . 2008

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